Human/Mouse/Rat UBE2N/Ubc13 Antibody

Catalog #: AF8197 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8197
AF8197-SP

Detection of Human, Mouse, and Rat UBE2N/Ubc13 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat UBE2N/Ubc13 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, rat, and mouse UBE2N/Ubc13 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human UbcH5b is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human UBE2N/Ubc13
Met1-Ile172
Accession # P61088

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat UBE2N/Ubc13 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HL-60 human acute promyelocytic leukemia cell line, Hepa 1-6 mouse hepatoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, C6 rat glioma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat UBE2N/Ubc13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8197) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBE2N/Ubc13 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UBE2N/Ubc13

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), is a 16 kDa member of the Ubiquitin-conjugating (E2) enzyme family. UBE2N/Ubc13 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin. UBE2N/Ubc13 localizes to both the nucleus and cytoplasm, and forms heterodimeric complexes with Uev1a/UBE2V1 and Mms2, both of which are catalytically inactive E2 enzyme variants. The UBE2N (Ubc13)/Uev1A complex is found in the cytoplasm and is important for inflammatory responses via Nuclear Factor kappa B (NF-kappa B) activation. In contrast, the UBE2N/Ubc13-Mms2 complex functions in the nucleus and is required for an efficient DNA damage response. Pathologically, UBE2N (Ubc13)/Uev1a complex-mediated NF-kappa B activation is required for the proliferation of diffuse large B-cell lymphoma cells. Human UBE2N/Ubc13 shares 100% and 99% amino acid sequence identity with its mouse and rat orthologs, respectively.

Entrez Gene IDs

7334 (Human); 93765 (Mouse); 116725 (Rat)

Alternate Names

bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; EC 6.3.2.19; MGC131857; MGC8489; UBC13; UbcH13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog