Home / SOD2/Mn-SOD / Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

Catalog #: DYC3419-2
23 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC3419-5
DYC3419-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Standard Curve
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Product Details
Citations (23)
FAQs
Supplemental Products
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
312.5 - 20,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total SOD2 / Mn-SOD in cell lysates. An immobilized capture antibody specific for SOD2 / Mn-SOD binds both phosphorylated and unphosphorylated SOD2 / Mn-SOD. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

ELISA View Larger

Standard Curve

N/A Detection of SOD2/Mn-SOD in HepG2 Cell Line Lysate. View Larger

Detection of SOD2/Mn-SOD in HepG2 Cell Line Lysate. Lysates prepared from HepG2 human hepatocellular carcinoma cells were incubated in wells coated with Human/Mouse/Rat Total SOD2/Mn-SOD Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. The same lysate and captured protein were electrophoresed, transferred to a PVDF membrane, and immunoblotted with a Human/Mouse/Rat SOD2 Polyclonal Antibody (R&D Systems®, Catalog # AF3419). Only the band corresponding to SOD2 was detected in captured material. To further determine specificity, recombinant human SOD1 and recombinant human SOD3 were assayed at 200 ng/mL and did not cross-react or interfere in the assay.

N/A Detection of SOD2/Mn-SOD in Cancer Cell Lysate. View Larger

Detection of SOD2/Mn-SOD in Cancer Cell Lysate. Lysates prepared from HepG2 human hepatocellular carcinoma cells, A172 human glioblastoma cells, HeLa human cervical epithelial carcinoma cells, Jurkat human acute T cell leukemia cells, and MDA-MB-453 human breast cancer cells were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of human/mouse/rat SOD2 detected by Western Blot.

N/A Detection of SOD2/Mn-SOD in Human PBMC Lysate. View Larger

Detection of SOD2/Mn-SOD in Human PBMC Lysate. Human peripheral blood mononuclear cells (PBMCs) were cultured for 24 hours in the absence or presence of 1 mM lipopolysaccharide (LPS). The lysates were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of human/mouse/rat SOD2 detected by Western Blot. Amounts of SOD2 as quantified by the Human/Mouse/Rat Total SOD2/Mn-SOD DuoSet® IC ELISA are consistent with the relative amounts of SOD2 determined by qualitative Western blot analysis.

N/A Detection of SOD2/Mn-SOD in Cell Lysate. View Larger

Detection of SOD2/Mn-SOD in Cell Lysate. Lysates prepared from DA3 mouse myeloma cells, L-929 mouse fibroblast cells, and PC-12 rat adrenal pheochromocytoma cells were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of SOD2 detected by Western Blot.

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SOD2/Mn-SOD

Superoxide Dismutases, originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Three mammalian isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SODs are typically soluble secreted or cytosolic proteins, but are also found in the mitochondria and extracellular matrix.

Any of three metals, manganese, iron, or copper, may be used in the active site of SOD and are indicative of cellular localization.MnSOD (SOD2) is found in the mitochondria of both prokaryotes and ukaryotes, whereas the cytosol of eukaryotes and prokaryotic organisms contains Cu/ZnSOD (SOD1) and FeSOD, respectively.

There have been several mutations identified in the Cu/ZnSOD (SOD1) gene in amyotrophic lateral sclerosis (ALS) patients, possibly suggesting a role for free radicals in this disease process. The mechanism of motor neuron degeneration that occurs in ALS, however, is unclear at this time. Several hypotheses have been explored for the role of mutant SOD1 and convincing evidence for the involvement of apoptosis has been presented, as well.

Long Name:
Superoxide Dismutase-2
Entrez Gene IDs:
6648 (Human); 20656 (Mouse); 24787 (Rat)
Alternate Names:
EC 1.15.1.1; indophenoloxidase B; IPOB; IPO-B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn SOD; Mn superoxide dismutase; MnSOD; MVCD6; SOD, Mitochodrial; SOD2; superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2, mitochondrial

Citations for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Effect of luteolin on oxidative stress and inflammation in the human osteoblast cell line hFOB1.19 in an inflammatory microenvironment
    Authors: Peng, Z;Zhang, W;Hong, H;Liu, L;
    BMC pharmacology & toxicology
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Combination therapy with probiotics and anti-PD-L1 antibody synergistically ameliorates sepsis in mouse model
    Authors: Sun, L;Fang, K;Yang, Z;
    Heliyon
    Species: Mouse
    Sample Types: Serum
  3. The Role of NF-kappaB and Bax/Bcl-2/Caspase-3 Signaling Pathways in the Protective Effects of Sacubitril/Valsartan (Entresto) against HFD/STZ-Induced Diabetic Kidney Disease
    Authors: M Mohany, MM Ahmed, SS Al-Rejaie
    Biomedicines, 2022-11-09;10(11):.
    Species: Rat
    Sample Types: Tissue Homogenates
  4. The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO2NPs) Using Caco-2 Cells
    Authors: OM David, KL Lategan, MF de Cortale, EJ Pool
    Biomolecules, 2022-09-21;12(10):.
    Species: Human
    Sample Types: Cell Lysates
  5. Toxic Effects of Endocrine Disruptor Exposure on Collagen-Induced Arthritis
    Authors: R D'Amico, E Gugliandol, M Cordaro, R Fusco, T Genovese, AF Peritore, R Crupi, L Interdonat, D Di Paola, S Cuzzocrea, D Impellizze, R Siracusa, R Di Paola
    Biomolecules, 2022-04-10;12(4):.
    Species: Mouse
    Sample Types: Cell Culture Supernatants
  6. Clostridioides difficile Toxin B Activates Group 3 Innate Lymphocytes
    Authors: RL Pope, A Chitrakar, P Sah, T Shadid, JD Ballard, LA Zenewicz
    Infection and Immunity, 2022-04-04;0(0):e0007322.
    Species: Rat
    Sample Types: Serum
  7. Minocycline relieves myocardial ischemia-reperfusion injury in rats by inhibiting inflammation, oxidative stress and apoptosis
    Authors: LQ Chen, WS Wang, SQ Li, JH Liu
    European review for medical and pharmacological sciences, 2022-04-01;26(8):3001-3009.
    Species: Rat
    Sample Types: Serum
  8. Miconazole Mitigates Acetic Acid-Induced Experimental Colitis in Rats: Insight into Inflammation, Oxidative Stress and Keap1/Nrf-2 Signaling Crosstalk
    Authors: IA Alsharif, HM Fayed, RF Abdel-Rahm, RM Abd-Elsala, HA Ogaly
    Biology, 2022-02-13;11(2):.
    Species: Rat
    Sample Types: Tissue Homogenates
  9. Clostridioides difficile Toxin B Activates Group 3 Innate Lymphocytes
    Authors: RL Pope, A Chitrakar, P Sah, T Shadid, JD Ballard, LA Zenewicz
    Infection and Immunity, 2022;0(0):e0007322.
    Species: Rat
    Sample Types: Serum
  10. Pharmacological Effects of Marine-Derived Enterococcus faecium EA9 against Acute Lung Injury and Inflammation in Cecal Ligated and Punctured Septic Rats
    Authors: HM Abuohashis, EH Zaghloul, AS El Sharkaw, EM Abbas, MM Ahmed, SS Al-Rejaie
    BioMed Research International, 2021-12-06;2021(0):5801700.
    Species: Rat
    Sample Types: Tissue Homogenates
  11. Mangiferin exert protective effects on joints of adjuvant-induced arthritis rats by regulating the MAPKs/NF-kappaB pathway of fibroblast-like synoviocytes
    Authors: R Wang, J Liu, Z Wang, X Wu, H Guo, X Jiao, H Zhang, C Qi, X Li
    International immunopharmacology, 2021-11-24;0(0):108352.
    Species: Rat
    Sample Types: Plasma
  12. AKR1C1 alleviates LPS?induced ALI in mice by activating the JAK2/STAT3 signaling pathway
    Authors: X Wang, B Yang, Y Li, J Luo, Y Wang
    Molecular Medicine Reports, 2021-09-30;24(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Aldosterone Excess Induced Mitochondria Decrease and Dysfunction via Mineralocorticoid Receptor and Oxidative Stress In Vitro and In Vivo
    Authors: CH Tsai, CT Pan, YY Chang, SY Peng, PC Lee, CW Liao, CT Shun, PT Li, VC Wu, CH Chou, IJ Tsai, CS Hung, YH Lin
    Biomedicines, 2021-08-02;9(8):.
    Species: Human, Rat
    Sample Types: Cell Lysates
  14. Mitochondrial dysfunction in airways and quadriceps muscle of patients with chronic obstructive pulmonary disease
    Authors: G Haji, CH Wiegman, C Michaeloud, MS Patel, K Curtis, P Bhavsar, MI Polkey, IM Adcock, KF Chung, COPDMAP co
    Respir Res, 2020-10-12;21(1):262.
    Species: Human
    Sample Types: Cell Lysates
  15. LCZ696 mitigates diabetic-induced nephropathy through inhibiting oxidative stress, NF-kappaB mediated inflammation and glomerulosclerosis in rats
    Authors: M Mohany, AZ Alanazi, F Alqahtani, OM Belali, MM Ahmed, SS Al-Rejaie
    PeerJ, 2020-06-19;8(0):e9196.
    Species: Rat
    Sample Types: Tissue Homogenates
  16. Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes
    Authors: LR Ling, DH Zheng, ZY Zhang, WH Xie, YH Huang, ZX Chen, XZ Wang, D Li
    Oncol Lett, 2020-02-17;19(4):2861-2869.
    Species: Mouse
    Sample Types: Serum
  17. Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice
    Authors: R Tan, Y He, S Zhang, D Pu, J Wu
    J Ovarian Res, 2019-07-19;12(1):65.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Effect of Telmisartan in the Oxidative Stress Components Induced by Ischemia Reperfusion in Rats
    Authors: SQ Rodríguez-, WA Trujillo-R, A Castillo-R, SE Totsuka-Su, TA Garcia-Cob, EG Cardona-Mu, AG Miranda-Dí, EJ Ramírez-Li, L García-Ben
    Oxid Med Cell Longev, 2019-07-02;2019(0):1302985.
    Species: Rat
    Sample Types: Tissue Homogenates
  19. Acute renal injury induced by endotoxic shock in rats is alleviated via PI3K/Nrf2 pathway
    Authors: CH Chuang, CK Yang, PH Wu, Y Zhang, PJ Yang
    Eur Rev Med Pharmacol Sci, 2018-08-01;22(16):5394-5401.
    Species: Rat
    Sample Types: Tissue Homogenates
  20. Protective effect and mechanism of rat recombinant S100 calcium-binding protein A4 on oxidative stress injury of rat vascular endothelial cells
    Authors: X Meng, X Gao, Z Zhang, X Zhou, L Wu, M Yang, K Wang, H Ren, B Sun, T Wang
    Oncol Lett, 2018-07-11;16(3):3614-3622.
    Species: Rat
    Sample Types: Cell Culture Supernates
  21. Protective Effects of Curcumin against Sodium Arsenite-induced Ovarian Oxidative Injury in a Mouse Model
    Authors: XN Wang, CJ Zhang, HL Diao, Y Zhang
    Chin. Med. J., 2017-05-05;130(9):1026-1032.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. Multigenerational Effects of Heavy Metals on Feeding, Growth, Initial Reproduction and Antioxidants in Caenorhabditis elegans
    PLoS ONE, 2016-04-26;11(4):e0154529.
    Species: Nematode
    Sample Types: Cell Culture Supernates
  23. Gastroprotective Effects of Astragaloside IV against Acute Gastric Lesion in Rats.
    Authors: Mao S, Yang G, Li W, Zhang J, Liang H, Li J, Zhang M
    PLoS ONE, 2016-02-04;11(2):e0148146.
    Species: Rat
    Sample Types: Tissue Homogenates

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