Human/Mouse RIPK4/RIP4 Antibody

Catalog #: AF10002 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF10002-100
AF10002-SP

Detection of Human RIPK4/RIP4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse RIPK4/RIP4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse RIPK4/RIP4 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human/mouse RIPK4/RIP4
Thr218-Thr450
Accession # P57078

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

A549 human lung carcinoma cell line, PANC‑1 human pancreatic carcinoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line

Immunohistochemistry

0.3-15 µg/mL

Immersion fixed paraffin-embedded sections of human kidney, and perfusion fixed frozen sections of mouse kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RIPK4/RIP4 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, PANC-1 human pancreatic carcinoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RIPK4/RIP4 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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RIPK4/RIP4 in Human Kidney. RIPK4/RIP4 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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RIPK4/RIP4 in Mouse Kidney. RIPK4/RIP4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RIPK4/RIP4

Receptor-interacting serine-threonine kinase 4 (RIPK4/PKK) is a serine/threonine protein kinase which interacts with protein kinase C-delta, but also activates NFkappaB, and interacts with the Wnt3a coreceptor LRP6 after Wnt3a stimulation. RIPK4 is required for keratinocyte differentiation and has broad expression in epithelial tissues. Down regulation of RIPK4 has been observed in squamous cell carcinomas, hepatocellular carcinoma, urothelial carcinoma and cervical carcinoma. Mutations in in the RIPK4 gene cause Popliteal pterygium syndrome, lethal type (PPS-L), CHAND syndrome and Bartsocas-Papas syndrome.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 4

Entrez Gene IDs

54101 (Human); 72388 (Mouse); 304053 (Rat)

Alternate Names

ANKK2; ANKRD3; ANKRD3MGC129993; ankyrin repeat domain 3; Ankyrin repeat domain-containing protein 3; DIKMGC129992; EC 2.7.11; EC 2.7.11.1; PKC-delta-interacting protein kinase; PKK; receptor-interacting serine/threonine-protein kinase 4; receptor-interacting serine-threonine kinase 4; RIP4; RIPK4; serine/threonine-protein kinase ANKRD3