Human/Mouse RND3 (RD1 Peptide) Antibody Summary
Cys197-Arg207
Accession # P61587
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human RND3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human RND3 (RD1 Peptide) Monoclonal Antibody (Catalog # MAB6618) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RND3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RND3
RND3, also known as RhoE, is an approximately 27 kDa member of the Rho family, small GTPase superfamily of molecules. It is 244 amino acids (aa) in length, and the mature chain extends from aa 1-241. RND3 is ubiquitously expressed and is involved in keratinocyte differentiation and stratification. RND3 overexpression results in cell size enlargement and an increase in the number of stratified cells. RND3 depletion results in hyperproliferation and delays initiation of keratinocyte differentiation. Human RND3 shares 100% aa sequence identity with mouse RND3.
Product Datasheets
Citations for Human/Mouse RND3 (RD1 Peptide) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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MicroRNA-200b inhibits epithelial-mesenchymal transition and migration of cervical cancer cells by directly targeting RhoE
Authors: YAN-XIANG CHENG, GAN-TAO CHEN, CHUANG CHEN, QI-FAN ZHANG, FENG PAN, MIN HU et al.
Molecular Medicine Reports
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TGF-beta1 stimulates human Tenon's capsule fibroblast proliferation by miR-200b and its targeting of p27/kip1 and RND3.
Authors: Tong J, Fu Y, Xu X, Fan S, Sun H, Liang Y, Xu K, Yuan Z, Ge Y
Invest Ophthalmol Vis Sci, 2014-04-28;55(4):2747-56.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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