Human/Mouse ROR gamma t/RORC2/NR1F3 APC-conjugated Antibody
Human/Mouse ROR gamma t/RORC2/NR1F3 APC-conjugated Antibody Summary
Met1-Gln100
Accession # P51449
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gammat/RORC2/NR1F3 APC-conjugated Monoclonal Antibody (Catalog # IC9125A) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). Quadrant markers were set based on control antibody staining (IC1051A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.
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Detection of ROR gamma t/RORC2/NR1F3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gammat/RORC2/NR1F3 APC-conjugated Monoclonal Antibody (Catalog # IC9125A) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (FAB554P). Quadrant markers were set based on control antibody staining (IC1051A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.
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Detection of ROR gamma t/RORC2/NR1F3 in HepG2 cells by Flow Cytometry. HepG2 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Monoclonal Antibody (Catalog # IC9125A, filled histogram) or isotype control antibody (Catalog # IC1051A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma t/RORC2/NR1F3
Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.
Product Datasheets
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