Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Catalog #: FAB3964A
1 Citation Datasheet / COA / SDS

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FAB3964A

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP by Flow Cytometry.

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Human/Mouse S1P5/EDG-8 APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse S1P₅/EDG‑8. Stains human and mouse S1P₅/EDG‑8 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 282503

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human S1P₅/EDG‑8
Met1-Asp398
Accession # Q9H228

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of S1P₅/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P₅/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human S1P₅/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P₅/EDG‑8 APC‑conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P5/EDG-8

S1P₅ is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P₅ is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P₅ show 96% and 97% aa identity with mouse and rat S1P₅, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P₅ is upregulated in large granular lymphocytic leukemias.

Long Name

Endothelial Differentiation Gene 8

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Alternate Names

EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1