Human/Mouse SALL4 Antibody

Name: Human/Mouse SALL4 Antibody
Brand: R&D Systems
SKU: MAB6374
Rating: 4.5 (2 reviews)

Catalog #: MAB6374 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6374
MAB6374-SP

Detection of Human SALL4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SALL4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse SALL4 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 651839

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SALL4
Lys96-Gly359
Accession # Q9UJQ4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.025 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human testis

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SALL4 by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.025 µg/mL of Mouse Anti-Human/Mouse SALL4 Monoclonal Antibody (Catalog # MAB6374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SALL4 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALL4

SALL4 (SAL-like protein 4) is a 1324 amino acid (aa) member of the SAL protein family that possess multiple C₂H₂-type zinc-fingers. It is a transcriptional repressor when associated with histone deacetylase and a transcriptional activator of the Wnt pathway in its native form. It contributes to generation of induced pluripotent stem cells and to axis formation during development. It is frequently overexpressed in germ cell tumors and acute myeloid leukemias. Mutations of human SALL4 can result in conditions such as Okihiro, Duane Radial Ray, or Holt-Oram syndromes. The region used as an immunogen is common to both SALL4 and the ubiquitous 617 aa isoform, SALL4B; it shares 76% aa identity between human and mouse.

Long Name

Sal-like 4

Entrez Gene IDs

57167 (Human); 99377 (Mouse)

Alternate Names

dJ1112F19.1; DRRS; HSAL4; MGC133050; SALL4; sal-like 4 (Drosophila); sal-like protein 4; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797; ZNF797sal (Drosophila)-like 4