Human/Mouse SIN3A Antibody

Catalog #: AF6115 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6115
AF6115-SP

Detection of Human SIN3A by Western Blot.

1 Image

All SIN3A Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SIN3A Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse SIN3A in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SIN3A
Tyr1187-Pro1273
Accession # Q96ST3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human SIN3A by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SIN3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6115) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SIN3A at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIN3A

SIN3A (Switch insensitive-3 A) is a nuclear, 140‑150 kDa member of the SIN3 family of co-repressors. SIN3A plays a key role in gene repression. Normally, genes are not expressed when chromatin is "closed", or condensed, precluding transcriptional activators from assembling on gene promoters. This state is achieved through histone deacetylation and DNA methylation, and SIN3A serves as a scaffold for multiple proteins, including deacetylases (HDAC1 and 2). Human SIN3A is 1273 amino acids (aa) in length. It contains three PAH (paired amphipathic helix) repeats (aa 141‑187, 322‑381 and 477‑523) and multiple phosphoserines and acetylated lysines. There are three potential splice variants. The first shows a 33 aa substtution for aa 124‑1273, while the second and third show eight aa substitutions for aa 337‑352, coupled to either a deletion of aa 1009‑1066, or a two aa substitution for aa 1008‑1065. Over aa 1187‑1273, human and mouse SIN3A show 100% identity in aa sequence.

Long Name

SIN3 Homolog A

Entrez Gene IDs

25942 (Human); 20466 (Mouse); 363067 (Rat)

Alternate Names

DKFZp434K2235; FLJ90319; Histone deacetylase complex subunit Sin3a; KIAA0700; paired amphipathic helix protein Sin3a; SIN3 homolog A, transcription regulator (yeast); SIN3 homolog A, transcriptional regulator (yeast); SIN3A; Transcriptional corepressor Sin3a; transcriptional co-repressor Sin3A; transcriptional regulator, SIN3A