Human/Mouse SOD3/EC-SOD Antibody Summary
Trp19-Ala240
Accession # P08294
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SOD3/EC‑SOD by Western Blot. Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human SOD3/EC-SOD Monoclonal Antibody (Catalog # MAB34201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOD3/EC-SOD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse SOD3/EC‑SOD by Simple WesternTM. Simple Western shows lysates of mouse aorta, loaded at 0.5 mg/ml. A specific band was detected for SOD3/EC‑SOD at approximately 41 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human SOD3/EC‑SOD Monoclonal Antibody (Catalog # MAB34201). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOD3/EC-SOD
Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring, but harmful, superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutase 3 (SOD3) also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. Human SOD3 shares ~64% sequence homology with mouse and rat SOD3. Like SOD1, SOD3 binds one Cu2+ and Zn2+ ions per subunit but differs in sequence and tissue distribution. SOD3 is a secretory protein and is synthesized with a putative 18-amino acid signal peptide preceding the 222 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP‑dependent oxidative system of neutrophils.
Product Datasheets
Citation for Human/Mouse SOD3/EC-SOD Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Human Neonatal Thymus Mesenchymal Stem/Stromal Cells and Chronic Right Ventricle Pressure Overload
Authors: Josue Chery, Shan Huang, Lianghui Gong, Shuyun Wang, Zhize Yuan, Joshua Wong et al.
Bioengineering (Basel)
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