Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody

Name: Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody
Brand: R&D Systems
SKU: AF464
Rating: 5 (2 reviews)

Catalog #: AF464
33 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF464
AF464-SP

Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot.

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All Sonic Hedgehog/Shh Antibodies

Product Details

Citations (33)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Sonic Hedgehog/Shh N‑Terminus in direct ELISAs and Western blots. In direct ELISAs, less than
15% cross‑reactivity with recombinant mouse (rm) Dhh N‑Terminus and rmIhh N-Terminus is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Sonic Hedgehog/Shh N‑Terminus
Cys25-Gly198 (Lys122Arg)
Accession # Q62226

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human stomach tissue and mouse stomach tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF464) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Sonic Hedgehog/Shh in Mouse Embryo. Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF464) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sonic Hedgehog/Shh

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Mouse and human Shh are 92% identical at the amino acid sequence level. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoeitic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases.

Mouse Shh cDNA encodes a 437 amino acid residue with a predicted 24 aa residue signal peptide that is cleaved to generate a 413 aa residue precursor protein. An autocatalytic reaction yields a 19 kDa amino-terminal domain Shh-N protein containing cholesterol and palmitate, and a 27 kDa carboxy-terminal domain Shh-C protein. The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase. Shh can act as both a short-range contact dependent factor and as a long-range, diffusible morphogen. At the cell surface, Shh activity is mediated by a multicomponent receptor complex involving the 12-pass transmembrane protein Patched (Ptc) which binds Shh with high affinity and Smoothened (Smo), a signaling seven transmembrane G-protein coupled receptor. In the absence of Shh, Ptc represses Smo activity. The binding of Shh to Ptc, releases the basal repression of Smo by Ptc (1‑5).

References

Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
Perrimon, N. (1995) Cell 80:517.
Weed, M. et al. (1997) Matrix Biol. 16:53.
Mullor, J. et al. (2002) Trends Cell Biol. 12:562.
Ingham, P. and A. McMahon (2001) Genes & Dev. 15:3059.

Entrez Gene IDs

6469 (Human); 20423 (Mouse)

Alternate Names

HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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Disrupting Hedgehog Cardin–Weintraub sequence and positioning changes cellular differentiation and compartmentalization in vivo
Authors: Philipp Kastl, Dominique Manikowski, Georg Steffes, Sabine Schürmann, Shyam Bandari, Christian Klämbt et al.
Development
Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development
Authors: Vitte J, Gao F, Coppola G et al.
Nat Commun
Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance.
Authors: Liu HX, Ermilov A, Grachtchouk M et al.
Dev Biol
Unique lingual expression of the Hedgehog pathway antagonist Hedgehog-interacting protein in filiform papillae during homeostasis and ectopic expression in fungiform papillae during Hedgehog signaling inhibition
Authors: Kumari A, Li L, Ermilov AN et al.
Developmental dynamics : an official publication of the American Association of Anatomists
Early taste buds are from Shh+ epithelial cells of tongue primordium in distinction from mature taste bud cells which arise from surrounding tissue compartments
Authors: Naomi Kramer, Guiqian Chen, Mohamed Ishan, Xiaogang Cui, Hong-Xiang Liu
Biochemical and Biophysical Research Communications
Proteolytic processing of palmitoylated Hedgehog peptides specifies the 3-4 intervein region of the Drosophila wing
Authors: Sabine Schürmann, Georg Steffes, Dominique Manikowski, Philipp Kastl, Ursula Malkus, Shyam Bandari et al.
eLife
Conserved cholesterol-related activities of Dispatched 1 drive Sonic hedgehog shedding from the cell membrane
Authors: Kristina Ehring, Dominique Manikowski, Jonas Goretzko, Jurij Froese, Fabian Gude, Petra Jakobs et al.
Journal of Cell Science
Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins
Authors: M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu
bioRxiv : the preprint server for biology, 2023-04-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition
Authors: Donnelly CR, Kumari A, Li L et al.
Cell and Tissue Research
Wnt Inhibitory Factor 1 Binds to and Inhibits the Activity of Sonic Hedgehog
Authors: K Kerekes, M Trexler, L Bányai, L Patthy
Cells, 2021-12-10;10(12):.
Species: Human
Sample Types: Protein
Applications: Western Blot

Sonic hedgehog signalling as a potential endobronchial biomarker in COPD
Authors: J Ancel, R Belgacemi, JM Perotin, Z Diabasana, S Dury, M Dewolf, A Bonnomet, N Lalun, P Birembaut, M Polette, G Deslée, V Dormoy
Respir. Res., 2020-08-07;21(1):207.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD
Authors: R Belgacemi, E Luczka, J Ancel, Z Diabasana, JM Perotin, A Germain, N Lalun, P Birembaut, X Dubernard, JC Mérol, G Delepine, M Polette, G Deslée, V Dormoy
EBioMedicine, 2019-12-23;51(0):102572.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell Niche
Authors: M Binder, P Chmielarz, PJ Mckinnon, LC Biggs, I Thesleff, A Balic
Stem Cells, 2019-06-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib
Authors: A Kumari, Y Yokota, L Li, RM Bradley, CM Mistretta
Sci Rep, 2018-11-01;8(1):16150.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling
PLoS Genet, 2016-11-28;12(11):e1006442.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells
Authors: Zhijian Zhang
Biochem. Biophys. Res. Commun, 2016-10-28;0(0):.
Species: Rat
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ICC, Western Blot

Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice
Authors: Vir B Singh
Sci Rep, 2016-05-31;6(0):26876.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Transcription factor 7-like 1 is involved in hypothalamo-pituitary axis development in mice and humans.
Authors: Gaston-Massuet C, McCabe M, Scagliotti V, Young R, Carreno G, Gregory L, Jayakody S, Pozzi S, Gualtieri A, Basu B, Koniordou M, Wu C, Bancalari R, Rahikkala E, Veijola R, Lopponen T, Graziola F, Turton J, Signore M, Mousavy Gharavy S, Charolidi N, Sokol S, Andoniadou C, Wilson S, Merrill B, Dattani M, Martinez-Barbera J
Proc Natl Acad Sci U S A, 2016-01-13;113(5):E548-57.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells.
Authors: Cai, Qing, Bonfanti, Paola, Sambathkumar, Rangaraj, Vanuytsel, Kim, Vanhove, Jolien, Gysemans, Conny, Debiec-Rychter, Maria, Raitano, Susanna, Heimberg, Harry, Ordovas, Laura, Verfaillie, Catherin
Stem Cells Transl Med, 2014-02-03;3(4):489-99.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation.
Authors: Mills, Lisa D, Zhang, Yaqing, Marler, Ronald J, Herreros-Villanueva, Marta, Zhang, Lizhi, Almada, Luciana, Couch, Fergus, Wetmore, Cynthia, Pasca di Magliano, Marina, Fernandez-Zapico, Martin E
J Biol Chem, 2013-03-12;288(17):11786-94.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Heparan sulfate-modulated, metalloprotease-mediated sonic hedgehog release from producing cells.
Authors: Dierker T, Dreier R, Petersen A, Bordych C, Grobe K
J. Biol. Chem., 2009-01-27;284(12):8013-22.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot

Involvement of the Edar signaling in the control of hair follicle involution (catagen).
Authors: Fessing MY, Sharova TY, Sharov AA, Atoyan R, Botchkarev VA
Am. J. Pathol., 2006-12-01;169(6):2075-84.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Neuroprotective properties of cultured neural progenitor cells are associated with the production of sonic hedgehog.
Authors: Rafuse VF, Soundararajan P, Leopold C, Robertson HA
Neuroscience, 2005-01-01;131(4):899-916.
Species: Chicken, Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Sonic hedgehog exerts distinct, stage-specific effects on tongue and taste papilla development.
Authors: Liu HX, MacCallum DK, Edwards C, Gaffield W, Mistretta CM
Dev. Biol., 2004-12-15;276(2):280-300.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium.
Authors: Mistretta CM, Liu HX, Gaffield W, MacCallum DK
Dev. Biol., 2003-02-01;254(1):1-18.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
Authors: van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
Gastroenterology, 2001-08-01;121(2):317-28.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition
Authors: Donnelly CR, Kumari A, Li L et al.
Cell and Tissue Research

Sonic hedgehog promotes autophagy of vascular smooth muscle cells
Authors: Haijie Li, Jingjing Li, Yuenan Li, Pavneet Singh, Liang Cao, Li-juan Xu et al.
American Journal of Physiology-Heart and Circulatory Physiology

Interaction between hedgehog signalling and PAX6 dosage mediates maintenance and regeneration of the corneal epithelium
Authors: Romana Kucerova, Natalie Dorà, Richard L. Mort, Karen Wallace, Lucy J. Leiper, Christina Lowes et al.
Mol Vis

Sonic Hedgehog acts as a macrophage chemoattractant during regeneration of the gastric epithelium
Authors: Chakrabarti J, Dua-Awereh M, Schumacher M et al.
NPJ Regenerative medicine

Increased activity of mesenchymal ALK2‐BMP signaling causes posteriorly truncated microglossia and disorganization of lingual tissues
Authors: Mohamed Ishan, Guiqian Chen, Chenming Sun, Shi‐You Chen, Yoshihiro Komatsu, Yuji Mishina et al.
genesis

Increased Programmed Death-Ligand 1 is an Early Epithelial Cell Response to Helicobacter pylori Infection
Authors: Holokai L, Chakrabarti J, Broda T et al.
PLoS Pathog.

Separate and distinctive roles for Wnt5a in tongue, lingual tissue and taste papilla development
Authors: Hong-Xiang Liu, Ann S. Grosse, Ken Iwatsuki, Yuji Mishina, Deborah L. Gumucio, Charlotte M. Mistretta
Developmental Biology