Human/Mouse SR140 Antibody

Catalog #: MAB9897 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9897-100
MAB9897-SP

Detection of Human and Mouse SR140 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SR140 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human SR140 in direct ELISAs. Detects human and mouse SR140 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 983615

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human SR140 Peptide
Accession # O15042

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse SR140 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse SR140 Monoclonal Antibody (Catalog # MAB9897) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR140 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SR140

SR140, also known as U2-associated protein SR140 (U2SURP) is a 118 kDa protein located in the nucleus. SR140 belongs to the splicing factor SR family and has 3 known isoforms that are produced by alternative splicing. The protein amino acid sequence is highly conserved across species and can be heavily phosphorylated, leading to a shift in observed molecular weight.

Long Name

U2-Associated Protein SR140

Entrez Gene IDs

23350 (Human); 67958 (Mouse); 315903 (Rat)

Alternate Names

140 kDa Ser/Arg-rich domain protein; FSAPa; KIAA0332; SR140; U2 snRNP-associated SURP domain containing; U2 snRNP-associated SURP motif-containing protein; U2-associated protein SR140; U2-Associated SR140 Protein; U2SURP