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Human/Mouse SSEA-3 Antibody

Catalog #: MAB1434
24 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1434
MAB1434-SP
SSEA‑3 in BG01V Human Stem Cells.
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-3 (4, 5).
Source
Monoclonal Rat IgM Clone # MC-631
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Four to eight cell stage mouse embryos
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SSEA-3 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

SSEA‑3 in BG01V Human Stem Cells. SSEA-3 was detected in immersion fixed non-permeabilized BG01V human embryonic stem cells using Human/Mouse SSEA-3 Monoclonal Antibody (Catalog # MAB1434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry Detection of Canine SSEA-3 by Flow Cytometry View Larger

Detection of Canine SSEA-3 by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine SSEA-3 by Flow Cytometry View Larger

Detection of Canine SSEA-3 by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine Human/Mouse SSEA-3 Antibody by Flow Cytometry View Larger

Detection of Canine Human/Mouse SSEA-3 Antibody by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine Human/Mouse SSEA-3 Antibody by Flow Cytometry View Larger

Detection of Canine Human/Mouse SSEA-3 Antibody by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-3

SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

References
  1. Zhou, D. et al. (2000) J. Biol. Chem. 275:22631.
  2. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  3. Draper, J.S. et al. (2002) J. Anat. 200:249.
  4. Shevinsky, L.H. et al. (1982) Cell 30:697.
  5. Kannagi, R. et al. (1983) EMBO J. 2:2355.
Long Name
Stage-specific Embryonic Antigen-3
Alternate Names
SSEA3; SSEA-3; Stage specific embryonic antigen 3; Stage-specific embryonic antigen 3

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Citations for Human/Mouse SSEA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Accelerating skin wound healing by M-CSF through generating SSEA-1 and -3 stem cells in the injured sites
    Authors: Yunyuan Li, Reza Baradar Jalili, Aziz Ghahary
    Scientific Reports
  2. Derivation of trophoblast stem cells from human expanded potential stem cells
    Authors: Shao Xu, Sidong Wang, Timothy Theodore Ka Ki Tam, Pengtao Liu, Degong Ruan
    STAR Protocols
  3. Generation and characterization of integration-free induced pluripotent stem cells from patients with autoimmune disease
    Authors: Mi-Young Son, Mi-Ok Lee, Hyejin Jeon, Binna Seol, Jung Hwa Kim, Jae-Suk Chang et al.
    Experimental & Molecular Medicine
  4. High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
    Authors: Hung, TH;Huang, Y;Yeh, CT;Yeh, CN;Yu, J;Lin, CC;Chiou, SP;Chiang, PY;Hung, JT;Yu, AL;
    Biomedical journal
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Flow Cytometry
  5. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate
    Authors: WS Fagg, N Liu, U Braunschwe, KL Pereira de, X Chen, FS Ditmars, SG Widen, JP Donohue, K Modis, WK Russell, JH Fair, MT Weirauch, BJ Blencowe, MA Garcia-Bla
    Nucleic Acids Research, 2022-05-20;50(9):5313-5334.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Induced pluripotent stem cells from subjects with Lesch-Nyhan disease
    Authors: DJ Sutcliffe, AR Dinasarapu, JE Visser, JD Hoed, F Seifar, P Joshi, I Ceballos-P, T Sardar, EJ Hess, YV Sun, Z Wen, ME Zwick, HA Jinnah
    Scientific Reports, 2021-04-19;11(1):8523.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Endoderm and Hepatic Progenitor Cells Engraft in the Quiescent Liver Concurrent with Intrinsically Activated Epithelial-to-Mesenchymal Transition
    Authors: WS Fagg, N Liu, I Patrikeev, OA Saldarriag, M Motamedi, VL Popov, HL Stevenson, JH Fair
    Cell Transplantation, 2021-01-01;30(0):9636897219937.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing
    Authors: H Jeon, JY Kim, JK Choi, E Han, CL Song, J Lee, YS Cho
    J Clin Med, 2018-11-20;7(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Progesterone Receptor Membrane Component 1 suppresses the p53 and Wnt/?-catenin pathways to promote human pluripotent stem cell self-renewal
    Authors: JY Kim, SY Kim, HS Choi, MK Kim, HM Lee, YJ Jang, CJ Ryu
    Sci Rep, 2018-02-14;8(1):3048.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma
    Authors: HH Kuo, RJ Lin, JT Hung, CB Hsieh, TH Hung, FY Lo, MY Ho, CT Yeh, YL Huang, J Yu, AL Yu
    Sci Rep, 2017-09-07;7(1):10750.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status
    Authors: Y Miyagoe-Su, T Nishiyama, M Nakamura, A Narita, F Takemura, S Masuda, N Minami, K Murayama, H Komaki, YI Goto, S Takeda
    Stem Cells Int, 2017-04-12;2017(0):7906843.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017-03-07;20(0):105-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016-08-10;6(0):31063.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  14. A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
    Authors: Matsumoto S, Nakao H, Kawabe K, Nonaka M, Toyoda H, Takishima Y, Kawabata K, Yamaguchi T, Furue M, Taki T, Okumura T, Yamazaki Y, Nakaya S, Kawasaki N, Kawasaki T
    J Biol Chem, 2015-06-22;290(33):20071-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  15. B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
    Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
    Stem Cells, 2014-10-01;32(10):2626-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012-08-31;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
    Authors: Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A
    Am J Obstet Gynecol, 2009-12-24;202(2):193.e1-193.e1.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  18. Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells.
    Authors: Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA
    Cell Stem Cell, 2009-04-03;4(4):359-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008-10-08;456(7220):344-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Secreted proteoglycans directly mediate human embryonic stem cell-basic fibroblast growth factor 2 interactions critical for proliferation.
    Authors: Levenstein ME, Berggren WT, Lee JE, Conard KR, Llanas RA, Wagner RJ, Smith LM, Thomson JA
    Stem Cells, 2008-09-18;26(12):3099-107.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008-05-01;215(2):329-36.
    Species: Equine
    Sample Types: Whole Cells
    Applications: ICC
  22. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008-04-03;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008-03-06;26(6):1436-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Generation of Human‐Induced Pluripotent Stem Cells From Anterior Cruciate Ligament
    Authors: Steven Woods, Nicola Bates, Sara L. Dunn, Ferdinand Serracino‐Inglott, Tim E. Hardingham, Susan J. Kimber
    Journal of Orthopaedic Research

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Reviews for Human/Mouse SSEA-3 Antibody

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Human/Mouse SSEA-3 Antibody
By Julieta O'Flaherty on 12/11/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

I used it to check for SSEA-3 in my iPSCs- beautiful staining

Immunocytochemistry/Immunofluorescence Human/Mouse SSEA-3 Antibody MAB1434
Human/Mouse SSEA-3 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human/Mouse SSEA-3 Antibody MAB1434
Human/Mouse SSEA-3 Antibody
By Carly Garrison on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human/Mouse SSEA-3 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human
Human/Mouse SSEA-3 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human