Human/Mouse SSEA-4 Biotinylated Antibody

Name: Human/Mouse SSEA-4 Biotinylated Antibody
Brand: R&D Systems
SKU: BAM1435
Rating: 5 (1 reviews)

Catalog #: BAM1435
1 Citation 1 Review Datasheet / COA / SDS

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BAM1435

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-4 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Recognizes a carbohydrate epitope of SSEA-4 (1, 2).

Source

Monoclonal Mouse IgG₃ Clone # MC-813-70

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

2120Ep human embryonal carcinoma cell line

Formulation

Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

NTera‑2 human testicular embryonic carcinoma cell line

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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SSEA‑4 in BG01V Human Stem Cells. SSEA‑4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA‑4 Biotinylated Monoclonal Antibody (Catalog # BAM1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References

Shevinsky, L.H. et al. (1982) Cell 30:697.
Kannagi, R. et al. (1983) EMBO J. 2:2355.
Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
Draper, J.S. et al. (2002) J. Anat. 200:249.

Long Name

Stage-specific Embryonic Antigen-4

Alternate Names

SSEA4; SSEA-4; SSEA-4;stage-specific embryonic antigen 4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse SSEA-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm.
Authors: Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S
Stem Cells, 2008-01-31;26(4):874-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC