Human/Mouse STAT4 Antibody

Catalog #: PA-ST4
1 Citation Datasheet / COA / SDS

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PA-ST4

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All STAT4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse STAT4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and murine STAT4. Does not cross-react with any of the other STAT proteins.

Source

Polyclonal Rabbit Serum

Formulation

Lyophilized from 0.2 mL antiserum containing 0.02% sodium azide.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:2000 dilution

STAT4 immunoprecipitated from lysates of human peripheral blood lymphocytes

Immunoprecipitation

3 µg/500 µg cell lysate

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation

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Immunoprecipitation of Human STAT4. STAT4 was immunoprecipitated from lysates (1 x 10⁶- 10⁷cells) of human peripheral blood lymphocytes (PBL) following incubation with 3 µg Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4) for 1 hour at 2 - 8 ºC. STAT4-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated STAT4 was detected by Western blot using a 1:2000 dilution of Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4). View our recommended buffer recipes for immunoprecipitation.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.

Long Name

Signal Transducer and Activator of Transcription 4

Entrez Gene IDs

6775 (Human); 20849 (Mouse)

Alternate Names

signal transducer and activator of transcription 4; SLEB11; STAT4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse STAT4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages.
Authors: Liao W, Lin JX, Wang L, Li P, Leonard WJ
Nat. Immunol., 2011-04-24;12(6):551-9.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: ChIP