Human/Mouse SUZ12 Antibody Summary
Thr131-Asn305
Accession # Q15022
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SUZ12 by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. Gels were loaded with 40 µg of cytoplasmic (Cyto) and 20 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse SUZ12 Monoclonal Antibody (Catalog # MAB4184) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SUZ12 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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SUZ12 in D3 Mouse Cell Line. SUZ12 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse Anti-Human/Mouse SUZ12 Monoclonal Antibody (Catalog # MAB4184) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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Immunoprecipitation of Human SUZ12. SUZ12 was immunoprecipitated from 400 µg of HeLa human cervical epithelial carcinoma cell line lysates using 4 µg of Mouse Anti-Human/Mouse SUZ12 Monoclonal Antibody (Catalog # MAB4184) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). HeLa lysates or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated SUZ12 was detected by Western blot under reducing conditions using 0.1 µg/mL Sheep Anti-Human SUZ12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4184) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SUZ12
SUZ12 (suppressor of zeste 12; also known as Joined to JazF1) is a 90-95 kDa member of the Polycomb group of transcriptional regulators. It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. Human SUZ12 is 739 amino acids (aa) in length. It contains one C2H2-type zinc finger region (aa 44-8471) and a C-terminal VEFS-box (aa 563-639). There is one potential splice form that shows a deletion of 51 aa over aa 129-197. Human SUZ12 shares 90% and 99% aa identity with mouse and dog SUZ12, respectively. Over the immunogen sequence in MAB4184, aa identity between human and mouse is 99%.
Product Datasheets
Citation for Human/Mouse SUZ12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Regulation of senescence escape by the cdk4-EZH2-AP2M1 pathway in response to chemotherapy
Authors: M Le Duff, J Gouju, B Jonchère, J Guillon, B Toutain, A Boissard, C Henry, C Guette, E Lelièvre, O Coqueret
Cell Death Dis, 2018-02-07;9(2):199.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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