Human/Mouse TOR PE-conjugated Antibody

Name: Human/Mouse TOR PE-conjugated Antibody
Brand: R&D Systems
SKU: IC1537P
Price: 309 USD

Catalog #: IC1537P Datasheet / COA / SDS

Discontinued Product

IC1537P has been discontinued.

View all TOR products.

Detection of TOR in Human PBMCs by Flow Cytometry.

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Human/Mouse TOR PE-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human TOR in direct ELISAs. Detects human and mouse TOR in flow cytometry.

Source

Monoclonal Rat IgG_2A Clone # 303728

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TOR
Phe1720-Ala2020
Accession # P42345

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TOR antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of TOR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with Anti-CD3, Anti-CD28, Recombinant Human TGF-beta 1 (Catalog # 240-B), and Recombinant Human IL-2 (Catalog # 202-IL) were stained with Rat Anti-Human/Mouse TOR PE-conjugated Monoclonal Antibody (Catalog # IC1537P) and Mouse Anti-Human CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of TOR antibody in Mouse Splenocytes antibody by Flow Cytometry.

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Detection of TOR in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with Anti-CD3, Anti-CD28, Recombinant Human TGF-beta 1 (Catalog # 240-B), and Recombinant Mouse IL-2 (Catalog # 402-ML) were stained with Rat Anti-Human/Mouse TOR PE-conjugated Monoclonal Antibody (Catalog # IC1537P) and Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TOR

TOR, also known as FRAP1, is a member of the PI 3-Kinase-related Kinase (PIKK) family. It is the protein target of Rapamycin, an anti-rejection drug used in transplantation and a promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-Kinase/Akt signal transduction pathway.

Long Name

Mammalian Target of Rapamycin

Entrez Gene IDs

2475 (Human); 287871 (Rat)

Alternate Names

EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/threonine-protein kinase mTOR; TOR