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Human/Mouse TORC2 Antibody

Catalog #: MAB6338
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6338
MAB6338-SP
Detection of Human and Mouse TORC2 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse TORC2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse TORC2 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 628430
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TORC2
Lys453-Ser613
Accession # Q53ET0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.01 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse TORC2 antibody by Western Blot. View Larger

Detection of Human and Mouse TORC2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, MDA-MB-453 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse TORC2 Monoclonal Antibody (Catalog # MAB6338) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TORC2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TORC2

TORC2, also known as CREB-regulated transcription co-activator 1 (crtc2), is an approximately 75 kDa protein that functions as a co‑activator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its O-glycosylation triggers TORC2-dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV-1 genes and the activation of latent EBV infections. Within aa 453‑613, human TORC2 shares 84% aa sequence identity with mouse TORC2.

Long Name
Transducer of Regulated CREB protein 1
Entrez Gene IDs
200186 (Human); 74343 (Mouse); 310615 (Rat)
Alternate Names
CREB regulated transcription coactivator 2; CREB-regulated transcription coactivator 2; CRTC2; TORC2; TORC-2; TORC2transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of CREB protein 2; Transducer of regulated cAMP response element-binding protein 2

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Citation for Human/Mouse TORC2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Osteocytes directly regulate osteolysis via MYD88 signaling in bacterial bone infection
    Authors: T Yoshimoto, M Kittaka, AAP Doan, R Urata, M Prideaux, RE Rojas, CV Harding, W Henry Boom, LF Bonewald, EM Greenfield, Y Ueki
    Nature Communications, 2022-11-04;13(1):6648.
    Species: Mouse
    Sample Types: Whole Cells

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