Human/Mouse TREM2 Antibody Summary
extracellular domain
Accession # Q99NH8
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
This antibody functions as a mouse TREM2 ELISA capture antibody when paired with Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of TREM2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0113).
Detection of TREM2 in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113).
Detection of TREM2 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113).
Detection of TREM2 in HEK293 Human Cell Line Transfected with eGFP and Either Human TREM2 or Human TREM1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP and either (A) human TREM2 or (B) human TREM1 was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). Quadrant markers were set based on control antibody staining (MAB0061).
TREM2 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line but is not detected in TREM2 knockout (KO) RAW 264.7 Mouse Cell Line cell line using Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips.
Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Detection of TREM2 in PBMC monocytes by Flow Cytometry PBMC monocytes were stained with Mouse Anti-Human CD11b/Integrin alpha M PE‑conjugated Monoclonal Antibody (Catalog # FAB16991P) and either (A) Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) or (B) isotype control antibody (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM2
TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.
Product Datasheets
Citations for Human/Mouse TREM2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Triggering Receptor Expressed on Myeloid Cell 2 R47H Exacerbates Immune Response in Alzheimer’s Disease Brain
Authors: Olena Korvatska, Kostantin Kiianitsa, Alexander Ratushny, Mark Matsushita, Neal Beeman, Wei-Ming Chien et al.
Frontiers in Immunology
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TREM2 promotes A? phagocytosis by upregulating C/EBP?-dependent CD36 expression in microglia
Authors: SM Kim, BR Mun, SJ Lee, Y Joh, HY Lee, KY Ji, HR Choi, EH Lee, EM Kim, JH Jang, HW Song, I Mook-Jung, WS Choi, HS Kang
Sci Rep, 2017-09-11;7(1):11118.
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Cyanidin-3-O-Glucoside Regulates the M1/M2 Polarization of Microglia via PPAR gamma and A beta 42 Phagocytosis Through TREM2 in an Alzheimer’s Disease Model
Authors: Sanjay, Jae-Ho Shin, Miey Park, Hae-Jeung Lee
Molecular Neurobiology
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LC3-Associated Endocytosis Facilitates beta-Amyloid Clearance and Mitigates Neurodegeneration in Murine Alzheimer's Disease.
Authors: Heckmann B L, Teubner B J W et al.
Cell
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Increased White Matter Inflammation in Aging- and Alzheimer’s Disease Brain
Authors: Divya Raj, Zhuoran Yin, Marjolein Breur, Janine Doorduin, Inge R. Holtman, Marta Olah et al.
Frontiers in Molecular Neuroscience
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Trem2 deficiency attenuates breast cancer tumor growth in lean, but not obese or weight loss, mice and is associated with alterations of clonal T cell populations
Authors: Pierro, EW;Cottam, MA;An, H;Lehmann, BD;Pietenpol, JA;Wellen, KE;Makowski, L;Rathmell, JC;Fingleton, B;Hasty, AH;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Plasma
Applications: ELISA Capture -
sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway
Authors: Arsenault, R;Marshall, S;Salois, P;Li, Q;Zhang, W;
Biology
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Target delivery of a PD-1-TREM2 scFv by CAR-T cells enhances anti-tumor efficacy in colorectal cancer
Authors: Chen, J;Zhu, T;Jiang, G;Zeng, Q;Li, Z;Huang, X;
Molecular cancer
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
BRI2-mediated regulation of TREM2 processing in microglia and its potential implications for Alzheimer's disease and related dementias
Authors: Yin, T;D'Adamio, L;
bioRxiv : the preprint server for biology
Species: Mouse, N/A, Transgenic Mouse
Sample Types: Tissue Homogenates, Protein
Applications: ELISA Detection -
Distinct myeloid population phenotypes dependent on TREM2 expression levels shape the pathology of traumatic versus demyelinating CNS disorders
Authors: Gao, H;Di, J;Clausen, BH;Wang, N;Zhu, X;Zhao, T;Chang, Y;Pang, M;Yang, Y;He, R;Wang, Y;Zhang, L;Liu, B;Qiu, W;Lambertsen, KL;Brambilla, R;Rong, L;
Cell reports
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
1,25-Dihydroxy Vitamin D3 Facilitates the M2 Polarization and ?-Amyloid Uptake by Human Microglia in a TREM2-Dependent Manner
Authors: Thu, VTA;Hoang, TX;Kim, JY;
BioMed research international
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
TREM2 expression on the microglia resolved lead exposure-induced neuroinflammation by promoting anti-inflammatory activities
Authors: Su, P;Zhang, J;Wu, J;Chen, H;Luo, W;Hu, M;
Ecotoxicology and environmental safety
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Skin basal cell carcinomas assemble a pro-tumorigenic spatially organized and self-propagating Trem2+ myeloid niche
Authors: Haensel, D;Daniel, B;Gaddam, S;Pan, C;Fabo, T;Bjelajac, J;Jussila, AR;Gonzalez, F;Li, NY;Chen, Y;Hou, J;Patel, T;Aasi, S;Satpathy, AT;Oro, AE;
Nature communications
Species: Human
Sample Types: Organoid
Applications: Neutralization -
Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome
Authors: X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen
Cell Reports, 2023-02-16;42(2):112133.
Species: Mouse
Sample Types: Cerebrospinal Fluid
Applications: ELISA Capture -
A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models
Authors: B van Lenger, L Zhan, D Xia, D Chan, D Joy, JI Park, D Tatarakis, M Calvert, S Hummel, S Lianoglou, ME Pizzo, R Prorok, E Thomsen, LM Bartos, P Beumers, A Capell, SS Davis, L de Weerd, JC Dugas, J Duque, T Earr, K Gadkar, T Giese, A Gill, J Gnörich, C Ha, M Kannuswamy, DJ Kim, ST Kunte, LH Kunze, D Lac, K Lechtenber, AW Leung, CC Liang, I Lopez, P McQuade, A Modi, VO Torres, HN Nguyen, I Pesämaa, N Propson, M Reich, Y Robles-Col, K Schlepckow, L Slemann, H Solanoy, JH Suh, RG Thorne, C Vieira, K Wind-Mark, K Xiong, YJY Zuchero, D Diaz, MS Dennis, F Huang, K Scearce-Le, RJ Watts, C Haass, JW Lewcock, G Di Paolo, M Brendel, PE Sanchez, KM Monroe
Nature Neuroscience, 2023-01-12;0(0):.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Flow Cytometry -
Naturally-aged microglia exhibit phagocytic dysfunction accompanied by gene expression changes reflective of underlying neurologic disease
Authors: AL Thomas, MA Lehn, EM Janssen, DA Hildeman, CA Chougnet
Scientific Reports, 2022-11-14;12(1):19471.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human iPSC co-culture model to investigate the interaction between microglia and motor neurons
Authors: BF Vahsen, E Gray, A Candalija, KML Cramb, J Scaber, R Dafinca, A Katsikoudi, Y Xu, L Farrimond, R Wade-Marti, WS James, MR Turner, SA Cowley, K Talbot
Scientific Reports, 2022-07-23;12(1):12606.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Microglial? signalling pathway deficits associated with the patient derived R47H TREM2 variants linked to AD indicate inability to activate inflammasome
Authors: K Cosker, A Mallach, J Limaye, TM Piers, J Staddon, SJ Neame, J Hardy, JM Pocock
Scientific Reports, 2021-06-25;11(1):13316.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Generation of cryopreserved macrophages from normal and genetically engineered human pluripotent stem cells for disease modelling
Authors: C Munn, S Burton, S Dickerson, K Bakshy, A Strouse, D Rajesh
PLoS ONE, 2021-04-22;16(4):e0250107.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Monocytic Infiltrates Contribute to Autistic-like Behaviors in a Two-Hit Model of Neurodevelopmental Defects
Authors: HR Chen, CW Chen, N Mandhani, JC Short-Mill, MR Smucker, YY Sun, CY Kuan
J Neurosci, 2020-10-30;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Resveratrol mediates mechanical allodynia through modulating inflammatory response via the TREM2-autophagy axis in SNI rat model
Authors: Y Wang, Y Shi, Y Huang, W Liu, G Cai, S Huang, Y Zeng, S Ren, H Zhan, W Wu
J Neuroinflammation, 2020-10-21;17(1):311.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Noncanonical function of an autophagy protein prevents spontaneous Alzheimer's disease
Authors: BL Heckmann, BJW Teubner, E Boada-Rome, B Tummers, C Guy, P Fitzgerald, U Mayer, S Carding, SS Zakharenko, T Wileman, DR Green
Sci Adv, 2020-08-14;6(33):eabb9036.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Receptor Recycling, Western Blot -
Alzheimer's-associated PLCgamma2 is a signaling node required for both TREM2 function and the inflammatory response in human microglia
Authors: BJ Andreone, L Przybyla, C Llapashtic, A Rana, SS Davis, B van Lenger, K Lin, J Shi, Y Mei, G Astarita, G Di Paolo, T Sandmann, KM Monroe, JW Lewcock
Nat. Neurosci., 2020-06-08;0(0):.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism
Authors: JK Götzl, M Brendel, G Werner, S Parhizkar, L Sebastian, G Kleinberge, AV Colombo, M Deussing, M Wagner, J Winkelmann, J Diehl-Schm, J Levin, K Fellerer, A Reifschnei, S Bultmann, P Bartenstei, A Rominger, S Tahirovic, ST Smith, C Madore, O Butovsky, A Capell, C Haass
EMBO Mol Med, 2019-06-01;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Detection -
Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model
Authors: L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen
Nat Commun, 2019-03-25;10(1):1365.
Species: Mouse, Transgenic Mouse
Sample Types: Cell Culture Supernates, Tissue Homogenates
Applications: ELISA Development (Capture) -
Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome
Authors: MA Russo, NT Fiore, C van Vreden, D Bailey, DM Santarelli, HM McGuire, B Fazekas de, PJ Austin
J Neuroinflammation, 2019-03-18;16(1):63.
Species: Human
Sample Types: Whole Cells
Applications: CyTof -
Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
Cell Rep, 2018-08-28;24(9):2300-2311.
Species: Human
Sample Types: Cell Culture Supernates
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A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells
Authors: MM Varnum, KA Clayton, A Yoshii-Kit, G Yonemoto, L Koro, S Ikezu, T Ikezu
J. Biol. Chem., 2017-05-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Interleukin-2 improves amyloid pathology, synaptic failure and memory in Alzheimer's disease mice
Authors: Sandro Alves
Brain, 2017-03-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Nuclear receptors license phagocytosis by trem2+ myeloid cells in mouse models of Alzheimer's disease.
Authors: Savage J, Jay T, Goduni E, Quigley C, Mariani M, Malm T, Ransohoff R, Lamb B, Landreth G
J Neurosci, 2015-04-22;35(16):6532-43.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
TREM-2 promotes macrophage survival and lung disease after respiratory viral infection.
Authors: Wu K, Byers D, Jin X, Agapov E, Alexander-Brett J, Patel A, Cella M, Gilfilan S, Colonna M, Kober D, Brett T, Holtzman M
J Exp Med, 2015-04-20;212(5):681-97.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: Flow Cytometry, IHC-P -
Triggering receptor expressed on myeloid cells 2 (TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke.
Authors: Kawabori M, Kacimi R, Kauppinen T, Calosing C, Kim J, Hsieh C, Nakamura M, Yenari M
J Neurosci, 2015-02-25;35(8):3384-96.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TREM2 splice isoforms generate soluble TREM2 species that disrupt long-term potentiation
Authors: Miguel Moutinho, Israel Coronel, Andy P. Tsai, Gonzalo Viana Di Prisco, Taylor Pennington, Brady K. Atwood et al.
Genome Medicine
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DSCR1 deficiency ameliorates the A beta pathology of Alzheimer's disease by enhancing microglial activity
Authors: Choi C, Kim H, Oh J et al.
Life science alliance
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Triggering receptor expressed on myeloid cells-2 expression in the brain is required for maximal phagocytic activity and improved neurological outcomes following experimental stroke
Authors: Kota Kurisu, Zhen Zheng, Jong Youl Kim, Jian Shi, Atsushi Kanoke, Jialing Liu et al.
Journal of Cerebral Blood Flow & Metabolism
FAQs
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What type of light chain does the Human/Mouse TREM-2 Antibody, Cat# MAB17291, clone # 237920 have?
This antibody has a kappa light chain.
Reviews for Human/Mouse TREM2 Antibody
Average Rating: 5 (Based on 2 Reviews)
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