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Human/Mouse UCP2 Antibody

Catalog #: AF4739
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4739
AF4739-SP
Detection of Human/Mouse UCP2 by Western Blot.
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Product Details
Citations (4)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse UCP2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse UCP2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) UCP1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human UCP2
Met1-Phe309
Accession # P55851
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse UCP2 antibody by Western Blot. View Larger

Detection of Human/Mouse UCP2 by Western Blot. Western blot shows lysates of mouse brown adipose tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human UCP1, UCP2, UCP3, and UCP4 (5 ng/lane) were included. A specific band for UCP2 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Simple Western Detection of Mouse UCP2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse UCP2 by Simple WesternTM. Simple Western lane view shows lysates of mouse brown adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for UCP2 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCP2

Mitochondrial Uncoupling Protein 2 (UCP2) is a 33 kDa member of the mitochondrial carrier protein family. UCP2 uncouples respiration from oxidative phosphorylation, principally in fat and skeletal muscle. Unlike UCP1, which generates heat in response to cold, UCP2 generates heat in response to dietary fluctuations. Human UCP2 is 309 amino acid (aa) in length. It contains six transmembrane domains (aa’s 11‑291) and is embedded in the inner mitochondrial membrane. Here, it dimerizes, forming a proton channel. There is one nucleotide binding site (aa 276‑298). Full‑length UCP2 shares 94%, 97% and 96% aa identity with porcine, canine and mouse UCP2, respectively.

Long Name
Uncoupling Protein 2
Entrez Gene IDs
7351 (Human); 22228 (Mouse); 54315 (Rat)
Alternate Names
mitochondrial uncoupling protein 2; SLC25A8; SLC25A8BMIQ4; Solute carrier family 25 member 8; UCP 2; UCP2; UCPH; uncoupling protein 2 (mitochondrial, proton carrier)

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Citations for Human/Mouse UCP2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Transcriptome analysis reveals brown adipogenic reprogramming in chemical compound-induced brown adipocytes converted from human dermal fibroblasts
    Authors: Y Takeda, T Yoshikawa, P Dai
    Scientific Reports, 2021-03-03;11(1):5061.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort.
    Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill
    PLoS ONE, 2014-01-08;9(1):e85436.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Upregulation of Angiotensin (1-7)-Mediated Signaling Preserves Endothelial Function Through Reducing Oxidative Stress in Diabetes
    Authors: Yang Zhang, Jian Liu, Jiang-Yun Luo, Xiao Yu Tian, Wai San Cheang, Jian Xu et al.
    Antioxidants & Redox Signaling
  4. Upregulation of UCP2 by Adiponectin: The Involvement of Mitochondrial Superoxide and hnRNP K.
    Authors: Zhou M, Xu A, Tam PK, Lam KS, Huang B, Liang Y, Lee IK, Wu D, Wang Y.
    PLoS One;7(2):e32349.

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