Human/Mouse Vav-1 Antibody

Name: Human/Mouse Vav-1 Antibody
Brand: R&D Systems
SKU: MAB3786
Rating: 2 (1 reviews)

Catalog #: MAB3786 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3786
MAB3786-SP

Detection of Human and Mouse Vav‑1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Vav-1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Vav‑1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 591529

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Vav‑1
Trp25-Leu228
Accession # P15498

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Vav‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Vav-1 Monoclonal Antibody (Catalog # MAB3786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vav-1

The Vav-1 proto-oncogene is a guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 promotes intracellular signaling by its multiple protein binding motifs. It is a cytosolic protein primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells.

Entrez Gene IDs

7409 (Human); 22324 (Mouse); 25156 (Rat)

Alternate Names

oncogene vav; proto-oncogene vav; vav 1 guanine nucleotide exchange factor; vav proto-oncogene; Vav1; Vav-1; vav-T; VAVvav 1 oncogene

Product Datasheets

Product Datasheet

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Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Human/Mouse Vav-1 Antibody

Average Rating: 2 (Based on 1 Review)

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Human/Mouse Vav-1 Antibody

By Anonymous on 06/06/2019

Application: WB Sample Tested: Lung single-cell suspension Species: Mouse

***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Western Blot Human/Mouse Vav-1 Antibody MAB3786