Human/Mouse VGF Antibody Summary
Thr554-Pro615
Accession # O15240
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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VGF in Human Brain. VGF was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human/Mouse VGF Monoclonal Antibody (Catalog # MAB6317) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VGF
Neurosecretory protein VGF (VGF) is a 65 kDa secreted protein that is expressed in the brain, where it may be involved in the regulation of cell-cell interactions or in synaptogenesis during maturation of the nervous system. Human VGF is synthesized as a 615 amino acid (aa) precursor that contains a 22 aa signal sequence and a 593 aa mature chain. The region including aa 353-447 is Asp/Glu-rich and thus an acidic region. Human VGF shares 87% aa sequence identity to mouse VGF.
Product Datasheets
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