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Human/Mouse WASF3/WAVE3 Antibody

Catalog #: AF5515
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5515
AF5515-SP
Detection of Human WASF3/WAVE3 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse WASF3/WAVE3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human/mouse WASF3/WAVE3 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human WASF3/WAVE3
Met1-Lys180
Accession # Q9UPY6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human WASF3/WAVE3 antibody by Western Blot. View Larger

Detection of Human WASF3/WAVE3 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, SH-SY5Y human neuroblastoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF3/WAVE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WASF3/WAVE3 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WASF3/WAVE3

WASF3/WAVE3 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 3) is a 70 kDa member of the SCAR/WAVE family of proteins. It is expressed in select diverse cell types such as neurons, platelets, and breast epithelium. Upon activation, WASF3/WAVE3 is phosphorylated on Tyr151/248/337/486 and, in complex with HSPC300, initiates actin polymerization. WASF3/WAVE3 also regulates MMP expression. Human WASF3/WAVE3 is 502 amino acids (aa) in length. It contains one coiled-coil region (aa 57‑93), three sequential poly-Pro regions (aa 304‑410) and one VPH domain (aa 440‑457) that binds to actin and Arp2/3. There is one isoform that shows a 36 aa substitution for aa 181‑239. Over aa 1‑180, human WASF3 shares 98% aa identity with mouse WASF3.

Long Name
Wiskott-Aldrich Syndrome Protein Family Member 3
Entrez Gene IDs
10810 (Human); 245880 (Mouse)
Alternate Names
Brush-1; KIAA0900WASP family Verprolin-homologous protein 3; Protein WAVE-3; SCAR3; SCAR3WASP family protein member 3; Verprolin homology domain-containing protein 3; WAS protein family, member 3; WASF3; WAVE3; WAVE3Brush-1; wisk

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Citations for Human/Mouse WASF3/WAVE3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex
    Authors: Wang, Y;Chiappetta, G;Guérois, R;Liu, Y;Romero, S;Boesch, DJ;Krause, M;Dessalles, CA;Babataheri, A;Barakat, AI;Chen, B;Vinh, J;Polesskaya, A;Gautreau, AM;
    Nature communications
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. PCARE requires coiled coil, RP62 kinase-binding and EVH1 domain-binding motifs for ciliary expansion
    Authors: Tess A V Afanasyeva, Yan-Ting Schnellbach, Toby J Gibson, Ronald Roepman, Rob W J Collin
    Human Molecular Genetics

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