Human/Mouse Wnt-3a Antibody Summary
Ser36-Gln75, Trp219-Arg269
Accession # P27467
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Wnt‑3a by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with mouse Wnt-3a. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB13242) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-3a at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Wnt3A in BG01V Human Stem Cells by Flow Cytometry. BGO1v human embryonic stem cells were stained with Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB13242, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-3a
Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).
- Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.
- Miller, J.R. (2001) Genome Biol. 3:3001.
- Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
- Lu, W et al. (2004) Cell 119:97.
- Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.
- Willert, K. et al. (2003) Nature 423:448.
- Komekado, H. et al. (2007) Genes Cells 12:521.
- Takada, R. et al. (2006) Dev. Cell 11:791.
- Entrez Accession # NP_001100475.
- Dunty, W.C. et al. (2008) Development 135:85.
- Lee, S.M.K. et al. (2000) Development 127:457.
- Takada, S. et al. (1994) Genes Dev. 8:174.
- Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
- Nusse, R. (2008) Cell Res. 18:523.
Product Datasheets
Citations for Human/Mouse Wnt-3a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Sevoflurane induces inflammation of microglia in hippocampus of neonatal rats by inhibiting Wnt/&beta-Catenin/CaMKIV pathway
Authors: F Wang, C Li, J Shao, J Ma
Journal of pharmacological sciences, 2021-03-16;146(2):105-115.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In�Vivo
Authors: Y Miao, A Ha, W de Lau, K Yuki, AJM Santos, C You, MH Geurts, J Puschhof, C Pleguezuel, WC Peng, R Senlice, C Piani, JW Buikema, OM Gbenedio, M Vallon, J Yuan, S de Haan, W Hemrika, K Rösch, LT Dang, D Baker, M Ott, P Depeille, SM Wu, J Drost, R Nusse, JP Roose, J Piehler, SF Boj, CY Janda, H Clevers, CJ Kuo, KC Garcia
Cell Stem Cell, 2020-08-19;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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This antibody demonstrates effective performance in ELISA detection, and we have successfully applied it in Western blot experiments. Additionally, it has shown reliable results across various cancer cell groups.
