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Human/Mouse Wnt-3a Antibody

Catalog #: MAB9025
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9025-SP
MAB9025-100
Topflash Reporter Activity Induced by Wnt-3a and Neutralization by Human/Mouse Wnt-3a Antibody.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Wnt-3a Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Wnt-3a in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 930769
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Wnt-3a
Ser19-Lys352
Accession # P27467
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize Wnt-3a induced Topflash reporter activity in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 0.15-0.9 μg/mL in the presence of 0.5 μg/mL Recombinant Mouse Wnt-3a.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Topflash Reporter Activity Induced by Wnt-3a and Neutralization by Human/Mouse Wnt-3a Antibody. View Larger

Topflash Reporter Activity Induced by Wnt-3a and Neutralization by Human/Mouse Wnt-3a Antibody. Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity elicited by Recombinant Mouse Wnt-3a (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB9025). The ND50 is typically 0.15-0.9 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-3a

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).

References
  1. Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.
  2. Miller, J.R. (2001) Genome Biol. 3:3001.
  3. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  4. Lu, W et al. (2004) Cell 119:97.
  5. Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.
  6. Willert, K. et al. (2003) Nature 423:448.
  7. Komekado, H. et al. (2007) Genes Cells 12:521.
  8. Takada, R. et al. (2006) Dev. Cell 11:791.
  9. Entrez Accession # NP_001100475.
  10. Dunty, W.C. et al. (2008) Development 135:85.
  11. Lee, S.M.K. et al. (2000) Development 127:457.
  12. Takada, S. et al. (1994) Genes Dev. 8:174.
  13. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  14. Nusse, R. (2008) Cell Res. 18:523.
Long Name
Wingless-type MMTV Integration Site Family, Member 3a
Entrez Gene IDs
89780 (Human); 22416 (Mouse)
Alternate Names
MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a

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Citations for Human/Mouse Wnt-3a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2
    Authors: YC Hsueh, RE Pratt, VJ Dzau, CP Hodgkinson
    Scientific Reports, 2023-03-09;13(1):3920.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation
    Authors: GFA de Moraes, E Listik, GZ Justo, CM Vicente, L Toma
    BMC molecular and cell biology, 2021-05-04;22(1):26.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, IHC, Immunoprecipitation, Western Blot
  3. Wnt signaling enhances macrophage responses to IL-4 and promotes resolution of atherosclerosis
    Authors: A Weinstock, K Rahman, O Yaacov, H Nishi, P Menon, CA Nikain, ML Garabedian, S Pena, N Akbar, BE Sansbury, SP Heffron, J Liu, G Marecki, D Fernandez, EJ Brown, KV Ruggles, SA Ramsey, C Giannarell, M Spite, RP Choudhury, P Loke, EA Fisher
    Elife, 2021-03-15;10(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. miR-600 Acts as a Bimodal Switch that Regulates Breast Cancer Stem Cell Fate through WNT Signaling
    Authors: R El Helou, G Pinna, O Cabaud, J Wicinski, R Bhajun, L Guyon, C Rioualen, P Finetti, A Gros, B Mari, P Barbry, F Bertucci, G Bidaut, A Harel-Bell, D Birnbaum, E Charafe-Ja, C Ginestier
    Cell Rep, 2017-02-28;18(9):2256-2268.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Activation of Wnt signaling promotes olaparib resistant ovarian cancer
    Authors: Tomomi M. Yamamoto, Alexandra McMellen, Zachary L. Watson, Jennifer Aguilera, Rebecca Ferguson, Elmar Nurmemmedov et al.
    Molecular Carcinogenesis

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