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Human/Mouse YY1 Antibody

Catalog #: MAB3784
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3784
MAB3784-SP
Detection of Human and Mouse YY1 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse YY1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human YY1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Rex-1/ZFP42 is observed.
Source
Monoclonal Mouse IgG2B Clone # 854847
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human YY1
His75-Gln275
Accession # P25490
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse YY1 antibody by Western Blot. View Larger

Detection of Human and Mouse YY1 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. Gels were loaded with 30 µg of U937 and 40 µg of C2C12 cytoplasmic (Cyto) extracts and 15 µg of U937 and 20 µg of C2C12 nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse YY1 Monoclonal Antibody (Catalog # MAB3784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YY1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: YY1

YY1 (Yin Yang 1) is a multifunctional transcription factor belonging to the GLI-Kruppel class of zing finger proteins. YY1 is essential for embryogenesis, apoptosis, differentiation, cell growth and proliferation. YY1 binds to the DNA consensus site CGCCATNTT. It can function as an initiator of transcription as well as activating or repressing transcription through protein complexes (e.g. histone deacetylases and histone acetyltransferases).

Long Name
Yin-Yang-1
Entrez Gene IDs
7528 (Human); 22632 (Mouse); 24919 (Rat)
Alternate Names
Delta transcription factor; DELTA; INO80SYY-1; NF-E1; NF-E1INO80 complex subunit S; transcriptional repressor protein YY1; UCRBP; Yin and Yang 1 protein; Yin and yang 1; YIN-YANG-1; YY1 transcription factor; YY1

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Citation for Human/Mouse YY1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization
    Authors: YW Dai, JK Ma, R Jiang, XL Zhan, SY Chen, LL Feng, Q Zhang, TB Liang, K Lv, GJ Yang, JF Lu, J Chen, XJ Lu
    Cell Reports, 2022-09-20;40(12):111361.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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