Human MS4A3 Fluorescein-conjugated Antibody

Catalog #: FAB6247F Datasheet / COA / SDS

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FAB6247F

Detection of MS4A3 in Human Whole Blood Monocytes by Flow Cytometry.

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Human MS4A3 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MS4A3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 489433

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human MS4A3
Accession # NP_006129

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MS4A3 in Human Whole Blood Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human MS4A3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB6247F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MS4A3

MS4A3, also known as HTm4, is an membrane protein that is a member of the MS4A family of four-transmembrane proteins that also includes CD20 and Fc epsilon RI beta. MS4A3 is a cell cycle regulator expressed in the perinuclear area of human basophils, eosinophils, early embryonic neural progenitors, splenic red pulp macrophages, monocytes and bile duct plus pancreatic duct epithelium. The C-terminal region of MS4A3 interacts with cdc-associated phosphatase (KAP), enhancing its activity on cyclin-dependent kinase 2 (cdk2) and facilitating G(0)/G(1) arrest. The combined non-membrane segments of the 214 amino acid (aa), 22-25 kDa human MS4A3 show 53% and 52% aa identity with corresponding regions of mouse and rat MS4A3, respectively.

Long Name

Membrane-spanning 4-domains, Subfamily A, Member 3 [Hematopoietic Cell-specific]

Entrez Gene IDs

932 (Human); 170813 (Mouse); 293753 (Rat)

Alternate Names

CD20 antigen homolog; CD20 antigen-like protein; CD20L; CD20LIgE receptor beta subunit; hematopoietic cell 4 transmembrane protein; Hematopoietic-specific transmembrane protein 4; HTM4; HTM4IgE receptor beta chain; membrane-spanning 4-domains subfamily A member 3; membrane-spanning 4-domains, subfamily A, member 3 (hematopoieticcell-specific); MS4A3