Human MSP beta Chain Antibody

Catalog #: MAB735
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB735
MAB735-SP

All MSP/MST1 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MSP beta Chain Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human (rh)MSP beta chain in direct ELISAs and Western blots. Shows no cross-reactivity with rhMSP alpha chain.

Source

Monoclonal Mouse IgG_2B Clone # 68801

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MSP beta chain
Val484-Gly711
Accession # P26927

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human MSP beta Chain (Catalog # 635-MS) under non-reducing conditions only

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.01-0.05 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human MSP (Catalog # 4306-MS) to immobilized Recombinant Human MSP R (Catalog # 1947-MS) coated at 1 μg/mL (100 µL/well). At 1 µg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSP/MST1

MSP, also known as hepatocyte growth factor-like protein (HGFl), and scatter factor-2 (SF2), is a member of the HGF family of growth factors. It is secreted as a single chain inactive precursor which is proteolytically activated to yield a biologically active disulfide-linked heterodimer of the alpha and beta chains. MSP induces keratinocyte proliferation, bone resorption, and cell migration and shape change in resident macrophages. Heterodimeric MSP binds to and activates the cell surface receptor tyrosine kinase, RON/STK, which is expressed on mature macrophages, keratinocytes, and vascular endothelium of wounded skin.

Long Name

Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]

Entrez Gene IDs

4485 (Human); 15235 (Mouse); 24566 (Rat)

Alternate Names

D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human MSP beta Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by: