Human MSP/MST1 beta Chain Biotinylated Antibody

Catalog #: BAM3521 Datasheet / COA / SDS

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BAM3521

All MSP/MST1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MSP/MST1 beta Chain Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human (rh)MSP in direct ELISAs and Western blots. When used in combination with the monoclonal capture antibody (Catalog # MAB352) in sandwich ELISAs, less than 0.001% cross-reactivity was observed with rhHGF, rhIL-9 R, rhMSP alpha or rmMSP R.

Source

Monoclonal Mouse IgG_2B Clone # 68801

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MSP beta chain
Accession # P26927

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human MSP (Catalog # 352-MS)
under non-reducing conditions only

Human MSP Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human MSP alpha Chain Antibody (Catalog # MAB352)

Standard: Recombinant Human MSP/MST1 Protein (Catalog # 352-MS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSP/MST1

MSP, also known as hepatocyte growth factor-like protein (HGFl), and scatter factor-2 (SF2), is a member of the HGF family of growth factors. It is secreted as a single chain inactive precursor which is proteolytically activated to yield a biologically active disulfide-linked heterodimer of the alpha and beta chains. MSP induces keratinocyte proliferation, bone resorption, and cell migration and shape change in resident macrophages. Heterodimeric MSP binds to and activates the cell surface receptor tyrosine kinase, RON/STK, which is expressed on mature macrophages, keratinocytes, and vascular endothelium of wounded skin.

Long Name

Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]

Entrez Gene IDs

4485 (Human); 15235 (Mouse); 24566 (Rat)

Alternate Names

D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2