Human MUC-1 PE-conjugated Antibody

Name: Human MUC-1 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB6298P

Catalog #: FAB6298P Datasheet / COA / SDS

Discontinued Product

FAB6298P has been discontinued.

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Detection of MUC‑1 in MCF‑7 Human Cell Line by Flow Cytometry.

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Human MUC-1 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MUC-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MUC-20, rhMUC-20S, or rhCA125/MUC-16 was observed.

Source

Monoclonal Mouse IgG_2B Clone # 604804

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MUC-1
Pro126-Arg145
Accession # P15941

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MUC‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-1 PE-conjugated Monoclonal Antibody (Catalog # FAB6298P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MUC-1

MUC-1 (Mucin-1; also PEM, PEMT, Episialin, Tumor-associated Mucin, and Carcinoma-associated Mucin) is a 240-450 kDa type I transmembrane glycoprotein. Human MUC-1 is 1255 amino acids (aa) in length and contains a 23 aa signal sequence, a 1135 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 74 aa cytoplasmic tail. The ECD consists of degenerate tandem repeats and a tandem repeat region, which makes up the major portion of the protein. Potential O‑glycosylation sites (serines and threonines) make up more than one-fourth of the amino acids. Splicing variants produce ten isoforms for human MUC-1. Human MUC-1 is 28% aa identical to mouse MUC-1.

Long Name

Mucin 1, Cell Surface-associated

Entrez Gene IDs

4582 (Human)

Alternate Names

Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

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