Human MUCDHL Antibody

Catalog #: AF3826 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3826
AF3826-SP

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Human MUCDHL Antibody Summary

Species Reactivity

Human

Specificity

Detects human MUCDHL in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse MUCDHL is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MUCDHL
Gln26-Ala434
Accession # Q9HBB8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MUCDHL

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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MUCDHL in Human Kidney. MUCDHL was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human MUCDHL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3826) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in collecting tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MUCDHL

Mucin and cadherin-like protein (MUCDHL), also known as μ-protocadherin, is a 110 kDa type I transmembrane protein that is both O- and N-glycosylated. It is a calcium-dependant cell adhesion protein in the cadherin superfamily. MUCDHL is found on intestinal and renal epithelium. Human MUCDHL extracellular domain (ECD) contains four cadherin domains (residues 71-459) plus several mucin domains (residues 540-645). Alternate splicing creates four isoforms that differ in their mucin domains. In the region used as immunogen, human and mouse MUCDHL share 63% amino acid sequence identity.

Long Name

Mucin And Cadherin-like

Entrez Gene IDs

53841 (Human); 72040 (Mouse); 171554 (Rat)

Alternate Names

cadherin-related family member 5; CDHR5; FLJ20219; MUCDHL; MUCDHLmucin-like protocadherin; Mucin and cadherin-like protein; mucin and cadherin-like; MUPCDH; MU-PCDH; mu-protocadherin