Human Mxi1 Antibody

Name: Human Mxi1 Antibody
Brand: R&D Systems
SKU: AF4185
Rating: 5 (1 reviews)

Catalog #: AF4185
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4185
AF4185-SP

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All Mxi1 Antibodies

Product Details

Citations (2)

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Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mxi1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mxi1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) MAD1, rhMAD3, and rhMAD4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Mxi1
Ala81-Ser228
Accession # P50539

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

Mxi1 in LNCaP Human Cell Line. Mxi1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4185) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mxi1

Mxi-1 (Max interactor 1) is a 26 kDa member of the bHLH-leucine zipper family, Myc superfamily of transcription factors. It is a widely expressed transcriptional repressor that heterodimerizes with Max and blocks Max-Myc gene activation. Human Mxi-1 is 228 amino acids (aa) in length. It contains a DNA binding motif
(aa 69‑80) and a bHLH domain (aa 81‑120). Multiple splice variants of Mxi-1 appear to exist. They may incorporate an alternate start site at Met37, a 92 aa substitution for the first 26 N-terminal amino acids, or a deletion of the C-terminal 59 or 74 amino acids. Over aa 81‑228, human Mxi-1 is 95% aa identical to mouse Mxi-1.

Long Name

MAX Interacting Protein 1

Entrez Gene IDs

4601 (Human); 17859 (Mouse); 25701 (Rat)

Alternate Names

BHLHC11; Class C basic helix-loop-helix protein 11; MAD2; MAX dimerization protein 2; MAX interacting protein 1; MAX interactor 1bHLHc11MAD2; max-interacting protein 1; Max-related transcription factor; MGC43220; MXD2; MXI; Mxi1; MXI-WR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Mxi1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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