Human MYCL1/L-Myc Antibody

Catalog #: MAB4050 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4050-100
MAB4050-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MYCL1/L-Myc Antibody Summary

Species Reactivity

Human

Specificity

Detects human MYCL1/L-MYC in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 803013

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MYCL1/L‑Myc
Gly16-Asn139
Accession # P12524

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MYCL1/L‑Myc Monoclonal Antibody(Catalog # MAB40501).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human MYCL1/L‑Myc ELISA Standard Curve. Recombinant Human MYCL1/L-Myc protein was serially diluted 2-fold and captured by Mouse Anti-Human MYCL1/L-Myc Monoclonal Antibody (Catalog # MAB40501) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human MYCL1/L-Myc Monoclonal Antibody (Catalog # MAB4050) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MYCL1/L-Myc

MYCL1 is a member of the MYC family that was isolated from small cell lung carcinoma. Like the other members of the MYC family, MYCL1 is a proto-oncogene transcription factor belonging to the helix-loop-helix basic leucine zipper (HLH bzip) family. With its heterodimeric partners, MYCL1 binds to the DNA consensus site 5’ CACGTG 3’. MYCL1 is implicated in controlling a wide range of cellular processes from cellular proliferation to apoptosis.

Long Name

v-Myc Myelocytomatosis Viral Oncogene Homolog 1, Lung Carcinoma Derived

Entrez Gene IDs

4610 (Human); 16918 (Mouse); 298506 (Rat)

Alternate Names

BHLHE38; bHLHe38myc-related gene from lung cancer; Class E basic helix-loop-helix protein 38; LMyc; L-Myc; LMYCl-myc-1 proto-oncogene; MYCL; MYCL1; protein L-Myc-1; v-myc avian myelocytomatosis viral oncogene homolog 1, lung carcinoma derived; v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian)