Human Myocilin APC-conjugated Antibody

Catalog #: FAB34461A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB34461A-100

Detection of Myocilin in HEK293 cell line transfected with hMYOC vs irrelevant HEK293 transfectant cells by Flow Cytometry.

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Human Myocilin APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Myocilin in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 297823

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Myocilin in HEK293 cell line transfected with hMYOC vs irrelevant HEK293 transfectant cells by Flow Cytometry. HEK293 cell line transfected with hMYOC vs irrelevant HEK293 transfectant cells were stained with Mouse Anti-Human Myocilin APC‑conjugated Monoclonal Antibody (Catalog # FAB34461A, filled histogram) or isotype control antibody, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Myocilin

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.

Entrez Gene IDs

4653 (Human)

Alternate Names

GLC1A; GLC1Amyocilin; GPOA; JOAG; JOAG1; MYOC; Myocilin; myocilin, trabecular meshwork inducible glucocorticoid response; TIGR; TIGRmutated trabecular meshwork-induced glucocorticoid response protein; Trabecular meshwork-induced glucocorticoid response protein