Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody Summary
Leu189-Leu741
Accession # Q09328
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human N‑Acetylglucosaminyltransferase V/MGAT5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N-Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
N‑Acetylglucosaminyltransferase V/MGAT5 in MCF‑7 Human Cell Line. N-Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: N-Acetylglucosaminyltransferase V/MGAT5
N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha 1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta 1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).
- Saito, H. et al. (1994) Biochem. Biophys. Res. Commun. 198:318.
- Lau, K.S. et al. (2007) Cell 198:123.
- Dennis, J.W. et al. (2002) Biochim. Biophys. Acta 1573:414.
- Granovsky, M. et al. (2008) Nat. Med. 7:1.
- Kim, Y.S. et al. (2008) Mol. Cell. Proteomics 7:1.
Product Datasheets
Citations for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
5
Citations: Showing 1 - 5
Filter your results:
Filter by:
-
Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan
Authors: Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka
Communications Biology
-
N-Acetylglucosaminyltransferase-V requires a specific non-catalytic luminal domain for its activity toward glycoprotein substrates
Authors: RF Osuka, T Hirata, M Nagae, M Nakano, H Shibata, R Okamoto, Y Kizuka
The Journal of Biological Chemistry, 2022-01-30;0(0):101666.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased beta1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding.
Authors: Priglinger C, Obermann J, Szober C, Merl-Pham J, Ohmayer U, Behler J, Gruhn F, Kreutzer T, Wertheimer C, Geerlof A, Priglinger S, Hauck S
PLoS ONE, 2016-01-13;11(1):e0146887.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
Authors: Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R
EMBO J, 2014-10-29;33(24):2890-905.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
N-acetylglucosaminyltransferase-V (GnT-V)-enriched small extracellular vesicles mediate N-glycan remodeling in recipient cells
Authors: Tetsuya Hirata, Yoichiro Harada, Koichiro M. Hirosawa, Yuko Tokoro, Kenichi G.N. Suzuki, Yasuhiko Kizuka
iScience
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Average Rating: 4 (Based on 1 Review)
Have you used Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
MGAT5 western blotting after MGAT5A transfection to 293 cells
(Figure Legend)
Ctrl: Control vector was transfected for 48 hrs.
MGAT5: MGAT5A vector was transfected for 48 hrs.
Above blots: human MGAT5 (80-100 kDa)
Lower blots: beta-actin (42-kDa)
Western blot condition: Tris buffer (0.5% Triton X)-lysed cell extract (15 ug each) was loaded with non-reducing condition (:No beta-MercaptoEthanol on loading buffer).
Primary Ab: anti-MGAT5 antibody (1 ug/ml) overnight at 4C
Secondary Ab: anti-mouse HRP antibody (1:10000 dilution) 90 minute at room temperature.
Development: SuperSignal West-Pico PLUS for 15 sec.
