Human N-Cadherin Antibody

Catalog #: MAB13883 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB13883-100
MAB13883-SP

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All N-Cadherin Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human N-Cadherin Antibody Summary

Species Reactivity

Human

Specificity

Detects human N- Cadherin in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 691721R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human N-Cadherin
Asp160-Ala724
Accession # P19022.4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human/Mouse/Rat N‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human N-Cadherin DuoSet ELISA Kit (Catalog # DY1388-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human N‑Cadherin ELISA Standard Curve. Recombinant Human N-Cadherin protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # MAB13883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: N-Cadherin

Neuronal Cadherin (N-Cadherin or NCAD), also known as Cadherin-2 (CDH2), is a 130 kDa type I membrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human N‑Cadherin is synthesized with a 25 amino acid (aa) signal peptide and a 134 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 565 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (3). Within the ECD, human N-Cadherin shares 98% aa sequence identity with mouse and rat N-Cadherin. In the nervous system, N-Cadherin mediates adhesion between the opposing faces of developing neuronal synapses and between Schwann cells and neuronal axons (4, 5). It interacts in cis or in trans homophilically and with the GluR2 subunit of neuronal AMPA receptors (1, 6). During synaptic maturation, its expression is lost from inhibitory terminals but maintained at excitatory terminals (5). ADAM10-mediated shedding of the N-Cadherin ECD alters cell-cell adhesion, synaptic development, and AMPA receptor activity (7, 8). N-Cadherin can also be cleaved at multiple additional sites within the intracellular or extracellular domains by Calpain, gamma ‑Secretase, and several MMPs (9-13). Cleavage of N‑Cadherin in atherosclerotic plaques contributes alternatively to vascular smooth muscle cell proliferation (MMP-9 and -12) or apoptosis (MMP‑7 (12, 13). Aberrant cell surface expression of the pro and mature forms of N-Cadherin in cancer results in increased tumor progression and invasiveness (14, 15). N‑Cadherin also mediates the adhesion between hematopoeitic progenitor cells and mesenchymal stromal cells of the bone marrow (16).

References

Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
Reid, R.A. and J.J. Hemperly (1990) Nucleic Acids Res. 18:5896.
Wanner, I.B. and P.M. Wood (2002) J. Neurosci. 22:4066.
Benson, D.L. and H. Tanaka (1998) J. Neurosci. 18:6892.
Saglietti, L. et al. (2007) Neuron 54:461.
Reiss, K. et al. (2005) EMBO J. 24:742.
Malinverno, M. et al. (2010) J. Neurosci. 30:16343.
Jang, Y.-N. et al. (2009) J. Neurosci. 29:5974.
Uemura, K. et al. (2006) Neurosci. Lett. 402:278.
Hartland, S.N. et al. (2009) Liver Int. 29:966.
Williams, H. et al. (2010) Cardiovasc. Res. 87:137.
Dwivedi, A. et al. (2009) Cardiovasc. Res. 81:178.
Maret, D. et al. (2010) Neoplasia 12:1066.
Tanaka, H. et al. (2010) Nat. Med. 16:1414.
Wein, F. et al. (2010) Stem Cell Res. 4:129.

Long Name

Neural Cadherin

Entrez Gene IDs

1000 (Human); 12558 (Mouse); 83501 (Rat)

Alternate Names

ACOGS; ARVD14; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325 antigen; CD325; CDH2; CDHN; CDw325; NCAD; N-cadherin 1; NCadherin; N-Cadherin; Neural cadherin; neural-cadherin