Human NAALADase-2/NAALAD2 Antibody

Catalog #: MAB7658 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7658
MAB7658-SP

Detection of Human NAALADase-2/NAALAD2 by Western Blot.

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Human NAALADase-2/NAALAD2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NAALADase-2/NAALAD2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse NAALADase-2 is observed and no cross-reactivity with recombinant human (rh) PSMA/FOLH1/NAALADase-1, rhNAALADase-like 2, or rhNAALADase-like 1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 756030

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-2/NAALAD2
Lys32-Leu740
Accession # Q9Y3Q0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NAALADase-2/NAALAD2 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NAALADase-2/NAALAD2 Monoclonal Antibody (Catalog # MAB7658) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NAALADase-2/NAALAD2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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NAALADase-2/NAALAD2 in Human Testis. NAALADase-2/NAALAD2 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human NAALADase-2/ NAALAD2 Monoclonal Antibody (Catalog # MAB7658) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAALADase-2/NAALAD2

N-acetylated-alpha-linked acidic dipeptidase 2 (NAALADase-2), also known as glutamate carboxypeptidase 3 (GCP3), is a 90-100 kDa type 2 transmembrane protein in the M28 peptidase family. It hydrolyzes beta-citrylglutamate (BCG) and N-acetyl-aspartylglutamate (NAAG) which are abundant dipeptides found in brain and testis. Mature human NAALADase-2 consists of a 7 amino acid (aa) cytoplasmic domain, a 24 aa transmembrane segment, and a 709 aa extracellular region (aa 32-740) that contains the peptidase domain. Over aa 32-740, human NAALADase-2 shares 89% aa sequence identity with mouse NAALADase-2.

Long Name

N-Acetylated Alpha-Linked Acidic Dipeptidase 2

Entrez Gene IDs

10003 (Human); 72560 (Mouse); 300384 (Rat)

Alternate Names

GCPIII; Glutamate Carboxypeptidase III; GPCIII; MGC116996; MGC26353; NAADALASE2; NAALAD2; NAALADase II; NAALADase2; NAALADase-2; N-acetylated alpha-linked acidic dipeptidase 2; N-acetylated alpha-linked acidic dipeptidase II; N-acetylated-alpha-linked acidic dipeptidase 2; N-acetylated-alpha-linked acidic dipeptidase II