Human NAALADase-like 1/NAALADL1 Antibody

Catalog #: AF5467 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5467
AF5467-SP

Detection of Human NAALADase-like 1/NAALADL1 by Western Blot.

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Human NAALADase-like 1/NAALADL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NAALADase-like 1/NAALADL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) NAALADase I, recombinant mouse NAALADase II, and rhNAALADase II is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-like 1/NAALADL1
Ala32-Leu740
Accession # NP_005459

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human NAALADase‑like 1/NAALADL1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NAALADase-like 1/NAALADL1 by Western Blot. Western blot shows lysates of human small intestine. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NAALADase-like 1/NAALADL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAALADase-like 1/NAALADL1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAALADase-like 1/NAALADL1

NAALADase-like1, also known as NAALADL1, (N-acetylated alpha -linked acidic dipeptidase like 1; also Ileal dipeptidylpeptidase) is a 100-115 kDa member of the peptidase M28 family of enzymes. It is found in the brush border of Ileal epithelium and demonstrates serine protease activity. Human NAALADL1 is a type II transmembrane glycoprotein that is 740 amino acids (aa) in length. It contains an extended extracellular domain (aa 29‑740) that shows a protease-associated region (aa 109‑335) with key catalytic residues at Ser617, Asp657 and His687. Multiple splice variants exist. Two show deletions of aa 161‑201 and 296‑330, respectively, while a third shows a 51 aa insertion after Pro359. Three show 18 to 42 aa substitutions after Pro502, with premature truncations after Ile522, Thr543 and Leu579. A final isoform shows a 17 aa substitution for aa 619‑740. Over aa 32‑740, human NAALADL1 shares 81% aa identity with mouse NAALADL1.

Long Name

N-Acetylated Alpha-Linked Acidic Dipeptidase-like 1

Entrez Gene IDs

10004 (Human); 381204 (Mouse); 83568 (Rat)

Alternate Names

I100; ileal dipeptidylpeptidase; NAALADase L; NAALADase like 1; NAALADASEL; NAALADase-like 1; NAALADL1 N-acetylated alpha-linked acidic dipeptidase-like 1; NAALADL1