Human NAIP Antibody

Catalog #: MAB829 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB829
MAB829-SP

Detection of Human NAIP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NAIP Antibody Summary

Species Reactivity

Human

Specificity

Detects human NAIP in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) NAIP or rmNAIP2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 541609

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human NAIP
Asn923-Val1148
Accession # Q13075

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NAIP by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NAIP and human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human NAIP Monoclonal Antibody (Catalog # MAB829) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NAIP at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry

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NAIP in Human Brain. NAIP was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Human NAIP Monoclonal Antibody (Catalog # MAB829) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAIP

Neuronal apoptosis inhibitory protein (NAIP; also Baculoviral IAP repeat-containing protein 1) is a 160 kDa member of the inhibitor of apoptosis family of proteins (also known as BIRC proteins). Human NAIP is 1403 amino acids (aa) in length. It contains three distinct regions: an N-terminal cluster of three baculoviral inhibitory repeat (BIR) domains, a central nucleotide binding oligomerization domain (NOD), and a C-terminal leucine-rich repeat (LRR) domain. Human NAIP shares 68% aa identity with mouse NAIP. NAIP is expressed in motor neurons, but not in sensory neurons. It is also expressed in the liver, placenta and to a lesser extent in the spinal cord. NAIP prevents motor neuron apoptosis, and defects in NAIP have been found in individuals with spinal muscular atrophy.

Long Name

Neuronal Apoptosis Inhibitor Protein

Entrez Gene IDs

4671 (Human); 17940 (Mouse); 191568 (Rat)

Alternate Names

baculoviral IAP repeat-containing 1; BIRC1; BIRC1baculoviral IAP repeat-containing protein 1; FLJ18088; FLJ42520; FLJ58811; NAIP; Neuronal apoptosis inhibitory protein; NLR family, apoptosis inhibitory protein; NLR family, BIR domain containing 1; NLRB1; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domaincontaining 1; psi neuronal apoptosis inhibitory protein; psiNAIP