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Human Nanog Biotinylated Antibody

Catalog #: BAF1997
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BAF1997
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Human Nanog Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects Nanog in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Nanog
Immunocytochemistry
5-15 µg/mL
Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).

References
  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name
Nanog Homeobox
Entrez Gene IDs
79923 (Human); 71950 (Mouse)
Alternate Names
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

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Citation for Human Nanog Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Diagnostic utility of novel stem cell markers SALL4, OCT4, NANOG, SOX2, UTF1, and TCL1 in primary mediastinal germ cell tumors.
    Authors: Liu A, Cheng L, Du J, Peng Y, Allan RW, Wei L, Li J, Cao D
    Am. J. Surg. Pathol., 2010-05-01;34(5):697-706.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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