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Human Nanog NorthernLights™ NL493-conjugated Antibody

Catalog #: NL1997G
2 Citations Datasheet / COA / SDS
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NL1997G
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nanog NorthernLights™ NL493-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
Source
Polyclonal Goat IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
NL493 (Excitation= 493 nm, Emission= 514 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).

References
  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name
Nanog Homeobox
Entrez Gene IDs
79923 (Human); 71950 (Mouse)
Alternate Names
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

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Citations for Human Nanog NorthernLights™ NL493-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Generation and characterization of a human induced pluripotent stem cell line heterozygous for a NOTCH1 mutation (NCHi014-A)
    Authors: Aljuhani, M;Choudhury, TZ;Yu, Y;Ye, S;Zhao, M;Garg, V;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  2. Derivation and High Engraftment of Patient-Specific Cardiomyocyte Sheet Using Induced Pluripotent Stem Cells Generated From Adult Cardiac Fibroblast
    Authors: Liying Zhang, Jing Guo, Pengyuan Zhang, Qiang Xiong, Steven C. Wu, Lily Xia et al.
    Circulation: Heart Failure

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