Human Nectin-2/CD112 APC-conjugated Antibody Summary
Gln32-Leu360
Accession # NP_002847
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Nectin‑2/CD112 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Nectin-2/CD112 APC-conjugated Monoclonal Antibody (Catalog # FAB2229A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Nectin-2/CD112
Nectins are a small family of Ca++-independent Immunoglobulin (Ig)-like Cell Adhesion Molecules (CAMs) that organize intercellular junctions (1). The nectin family has at least four members (nectin-1-4), all of which show alternate splicing (except for Nectin-4), a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Nectin-2 is a 60 or 65 kDa type I TM glycoprotein that is found on a variety of cell types (2, 3). It has two splice forms (4, 5). Nectin-2δ is a 65 kDa long form and is synthesized as a 538 amino acid precursor. It contains a 31 amino acid (aa) signal sequence, a 329 aa extracellular region, a 21 aa TM segment, and a 157 aa cytoplasmic domain. The extracellular region contains one N-terminal 85 aa V-type Ig domain and two 45-55 aa C2-type Ig domains. The V-domain is believed to mediate nectin binding to its ligands (6). The short, 60 kDa isoform of Nectin-2 (Nectin-2 alpha ) has the same signal sequence and extracellular domain as nectin-2δ, but differs in the TM and cytoplasmic region (4, 5). In this case, the cytoplasmic tail is only 94 aa in length. The human extracellular region shows 72% aa sequence identity with the equivalent region in mouse. Nectin-2 is known to bind the pseudorabies virus, and herpes simplex virus-2 (HSV-2), but not HSV-1. It does not bind poliovirus. As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell), followed by trans-dimers (across cells). Nectin-2 will not cis-dimerize with other nectins, but will cis-dimerize with its two splice forms. Notably, a Nectin-2 cis-dimer on one cell will heterodimerize with a Nectin-3 cis-dimer on another cell (1). Nectin-2 is found concentrated in adherens junctions, and exists on neurons, endothelial cells, epithelial cells and fibroblasts.
- Takai, Y. and H. Nakanishi, 2003, J. Cell Sci. 116:17.
- Bottino, C. et al. (2003) J. Exp. Med. 198:557.
- Pende, D. et al. (2005) Mol. Immunol. 42:463.
- Eberle, F. et al. (1995) Gene 159:267.
- Warner, M.S. et al. (1998) Virology 246:179.
- Struyf, F. et al. (2002) J. Virol. 76:12940.
Product Datasheets
Citations for Human Nectin-2/CD112 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
Cell Reports, 2022-09-27;40(13):111423.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
NKG2A and HLA-E define an alternative immune checkpoint axis in bladder cancer
Authors: Bérengère Salomé, John P. Sfakianos, Daniel Ranti, Jorge Daza, Christine Bieber, Andrew Charap et al.
Cancer Cell
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Fine tuning of the DNAM-1/TIGIT/ligand axis in mucosal T cells and its dysregulation in pediatric inflammatory bowel diseases (IBD)
Authors: S Battella, S Oliva, L Franchitti, R La Scaleia, A Soriani, S Isoldi, C Capuano, C Pighi, S Morrone, R Galandrini, A Santoni, G Palmieri
Mucosal Immunol, 2019-10-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Ubiquitin-proteasome pathway regulates Nectin2/CD112 expression and impairs NK cell recognition and killing
Authors: R Molfetta, ND Milito, B Zitti, M Lecce, C Fionda, M Cippitelli, A Santoni, R Paolini
Eur. J. Immunol., 2019-03-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells
Authors: B Zitti, R Molfetta, C Fionda, L Quatrini, H Stabile, M Lecce, V de Turris, MR Ricciardi, MT Petrucci, M Cippitelli, A Gismondi, A Santoni, R Paolini
Sci Rep, 2017-09-05;7(1):10445.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cultivation and characterization of pterygium as an ex vivo study model for disease and therapy
Authors: N Josifovska, DJ Szabó, R Nagymihály, Z Veréb, A Facskó, K Eriksen, MC Moe, G Petrovski
Cont Lens Anterior Eye, 2017-05-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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