Human Nectin-4 Alexa Fluor® 700-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Nectin-4

Nectin-4 is a 66-70 kDa member of the nectin family, Ig superfamily of molecules. Mature Nectin-4 is a 479 amino acid (aa) Type I transmembrane glycoprotein that contains one V-type and two C2-type Ig-like domains. Nectins mediate the Ca⁺⁺-independent cell adhesion in junctional complexes and connect to the Actin cytoskeleton via Afadin. Nectins are considered to first form loose cis-homodimers which aggregate and generate homooligomer in-trans, but preferentially generates Nectin-4:Nectin-1 heterooligomers. Cells known to express Nectin-4 include keratinocytes, transitional epithelium, CD49f⁺ CD24⁺ luminal mammary epithelium, tooth stratum intermedium cells, and respiratory pseudostratified ciliated columnar epithelium. It is also found on multiple carcinoma cell types. Nectin-4 can be cleaved to generate a 43-44 kDa soluble form that apparently appears only during tumorigenesis. Nectin-4 is also known to bind the measles virus, and possibly interact, indirectly or as a heterodimer, with the prolactin receptor. Over aa 27-351, human and mouse Nectin-4 share 90% aa sequence identity.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• Cell to Cell Adhesion Disassembly During EMT
• Epithelial Cell Markers and Intracellular Molecules
• Epithelial Cell Polarity
• Infectious Diseases
• Nectin and Nectin-like Binding Receptors and Ligands
• Nectins
• Search Immune Checkpoint Targets
• Surface Ectodermal Cells