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Human Nectin-4 Antibody

Catalog #: AF2659
22 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2659
AF2659-SP
Nectin‑4 in Human Placenta.
10 Images
Product Details
Citations (22)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nectin-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse Nectin-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Nectin‑4 (Catalog # 2659-N4)
Flow Cytometry
0.25 µg/106 cells
MCF‑7 human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Nectin‑4 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

Nectin‑4 in Human Placenta. Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human Nectin-4/PVRL4 by Western Blot View Larger

Detection of Human Nectin-4/PVRL4 by Western Blot Inhibition of Nectin-4 expression by gene silencing decreases cell proliferation in pancreatic cancer cells. (A,B) Capan-2 and BxPC-3 cells were transfected with Nectin-4 siRNA or control RNA. The relative expression of Nectin-4 was significantly reduced in both cells when transfected with Nectin-4 siRNA for up to 72 hours as determined by quantitative real-time PCR and Western blot analysis (n = 4 of each group). (C) Cell proliferation was significantly inhibited by Nectin-4 gene silencing in both cells after 72 hours incubation as determined by MTS assay (n = 6 of each group). *P < 0.001 versus control siRNA (Student’s t test). Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0144-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry View Larger

Detection of Nectin-4 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast adenocarcinoma cell line was stained with Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659, filled histogram) or control antibody (AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (F0107).

Western Blot Detection of Human Nectin-4/PVRL4 by Western Blot View Larger

Detection of Human Nectin-4/PVRL4 by Western Blot Knockdown expression and over-expression of Nectin-4 in human EC cell lines Eca-109 and TE-1. a The knockdown expression of Nectin-4 at mRNA level by using RNAi approach in human EC lines was confirmed by real-time RT-PCR, and the Nectin-4 mRNA expression level in LV-Nectin-4-shRNA group cells was significantly lower than that in LV-NC group cells (both in Eca-109 and in TE-1, P < 0.0001). b The decreased Nectin-4 protein expression after knockdown in human EC cell lines Eca-109 and TE-1, were confirmed by using western blot method. c The Nectin-4 protein expression level in LV-Nectin-4-shRNA group cells was significantly lower than that in LV-NC group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001). d The over-expression of Nectin-4 at mRNA level in human EC lines was confirmed by real-time RT-PCR, which showed that the increased Nectin-4 mRNA expression level in LV-Nectin-4-OE group cells compared with LV-Vector-Ctrl group cells (both in Eca-109 and in TE-1, P < 0.0001). e The increased Nectin-4 protein expression in human EC cell lines Eca-109 and TE-1 were confirmed by using western blot method. f The Nectin-4 protein expression level in LV-Nectin-4-OE group cells was significantly higher than that in LV-Vector-Ctrl group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human Nectin-4/PVRL4 by Immunohistochemistry View Larger

Detection of Human Nectin-4/PVRL4 by Immunohistochemistry Nectin-4 expression in EC tissues. a, b Positive staining of Nectin-4 could be found in the cytoplasm and on the membrane of cancer cells, while weak or negative staining of Nectin-4 was found in normal esophageal tissues. c The staining intensity of Nectin-4 in EC tissues was significantly higher than that in adjacent normal tissues (P < 0.0001). d The survival analysis showed that the overall survival rate of the patients with higher Nectin-4 expression was significantly poorer compared with those showing lower Nectin-4 expression (HR = 1.704, 95% CI 1.027–2.825, P = 0.039) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human Nectin-4/PVRL4 by Immunohistochemistry View Larger

Detection of Human Nectin-4/PVRL4 by Immunohistochemistry Nectin-4 expression in EC tissues. a, b Positive staining of Nectin-4 could be found in the cytoplasm and on the membrane of cancer cells, while weak or negative staining of Nectin-4 was found in normal esophageal tissues. c The staining intensity of Nectin-4 in EC tissues was significantly higher than that in adjacent normal tissues (P < 0.0001). d The survival analysis showed that the overall survival rate of the patients with higher Nectin-4 expression was significantly poorer compared with those showing lower Nectin-4 expression (HR = 1.704, 95% CI 1.027–2.825, P = 0.039) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence Nectin-4 expression in human pancreatic cancer tissues. (A,B) Tumor cell with high or low Nectin-4 expression. (C) There was limited expression of Nectin-4 in non-cancer tissues including islet cells, acinar cells and normal gland. Representative case of tumor Nectin-4 expression. Original magnification, ×200. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0144-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence Nectin-4 expression in human pancreatic cancer tissues. (A,B) Tumor cell with high or low Nectin-4 expression. (C) There was limited expression of Nectin-4 in non-cancer tissues including islet cells, acinar cells and normal gland. Representative case of tumor Nectin-4 expression. Original magnification, ×200. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0144-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human Nectin-4/PVRL4 by Western Blot View Larger

Detection of Human Nectin-4/PVRL4 by Western Blot Knockdown expression and over-expression of Nectin-4 in human EC cell lines Eca-109 and TE-1. a The knockdown expression of Nectin-4 at mRNA level by using RNAi approach in human EC lines was confirmed by real-time RT-PCR, and the Nectin-4 mRNA expression level in LV-Nectin-4-shRNA group cells was significantly lower than that in LV-NC group cells (both in Eca-109 and in TE-1, P < 0.0001). b The decreased Nectin-4 protein expression after knockdown in human EC cell lines Eca-109 and TE-1, were confirmed by using western blot method. c The Nectin-4 protein expression level in LV-Nectin-4-shRNA group cells was significantly lower than that in LV-NC group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001). d The over-expression of Nectin-4 at mRNA level in human EC lines was confirmed by real-time RT-PCR, which showed that the increased Nectin-4 mRNA expression level in LV-Nectin-4-OE group cells compared with LV-Vector-Ctrl group cells (both in Eca-109 and in TE-1, P < 0.0001). e The increased Nectin-4 protein expression in human EC cell lines Eca-109 and TE-1 were confirmed by using western blot method. f The Nectin-4 protein expression level in LV-Nectin-4-OE group cells was significantly higher than that in LV-Vector-Ctrl group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence Nectin-4 expression in human pancreatic cancer tissues. (A,B) Tumor cell with high or low Nectin-4 expression. (C) There was limited expression of Nectin-4 in non-cancer tissues including islet cells, acinar cells and normal gland. Representative case of tumor Nectin-4 expression. Original magnification, ×200. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0144-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Long Name
Poliovirus Receptor Related 4
Entrez Gene IDs
81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)
Alternate Names
EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; Nectin4; Nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4

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Citations for Human Nectin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Cap-dependent translational control of oncolytic measles virus infection in malignant mesothelioma
    Authors: Blake A. Jacobson, Ahad A. Sadiq, Shaogeng Tang, Joe Jay-Dixon, Manish R. Patel, Jeremy Drees et al.
    Oncotarget
  2. Nectin‐4 and p63 immunohistochemical expression in canine prostate tumourigenesis
    Authors: Leonardo Della Salda, Marcella Massimini, Mariarita Romanucci, Chiara Palmieri, Antonella Perillo, Valeria Grieco et al.
    Veterinary and Comparative Oncology
  3. Membranous Nectin-4 expression is a risk factor for distant relapse of T1-T2, N0 luminal-A early breast cancer
    Authors: R Lattanzio, R Ghasemi, F Brancati, R L Sorda, N Tinari, L Perracchio et al.
    Oncogenesis
  4. Measles Vaccine Strains for Virotherapy of Non–Small-Cell Lung Carcinoma
    Authors: Manish R. Patel, Blake A. Jacobson, Holly Belgum, Ahmad Raza, Ahad Sadiq, Jeremy Drees et al.
    Journal of Thoracic Oncology
  5. The Nectin-4/Afadin Protein Complex and Intercellular Membrane Pores Contribute to Rapid Spread of Measles Virus in Primary Human Airway Epithelia
    Authors: Brajesh K. Singh, Andrew L. Hornick, Sateesh Krishnamurthy, Anna C. Locke, Crystal A. Mendoza, Mathieu Mateo et al.
    Journal of Virology
  6. Immune response elicited in the tumor microenvironment upon rMV‐SLAMblind cancer virotherapy
    Authors: Kanako Moritoh, Koichiro Shoji, Yosuke Amagai, Tomoko Fujiyuki, Hiroki Sato, Misako Yoneda et al.
    Cancer Science
  7. Localization of nectin-2? at the boundary between the adjacent somata of the clustered cholinergic neurons and its regulatory role in the subcellular localization of the voltage-gated A-type K+channel Kv4.2 in the medial habenula
    Authors: H Shiotani, M Miyata, Y Itoh, S Wang, A Kaito, A Mizoguchi, M Yamasaki, M Watanabe, K Mandai, H Mochizuki, Y Takai
    J. Comp. Neurol., 2018-03-30;0(0):.
  8. Anti-tumor activity of a recombinant measles virus against canine lung cancer cells
    Authors: Tamura, K;Fujiyuki, T;Moritoh, K;Akimoto, H;Iizuka, K;Sato, H;Asano, K;Yoneda, M;Kai, C;
    Scientific reports
    Species: Xenograft
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. LRP6 Is a Functional Receptor for Attenuated Canine Distemper Virus
    Authors: V Gradauskai, M Inglebert, J Doench, M Scherer, M Dettwiler, M Wyss, N Shrestha, S Rottenberg, P Plattet
    MBio, 2023-01-16;0(0):e0311422.
    Species: Chlorocebus aethiops (green monkey)
    Sample Types: Whole Cells
    Applications: ICC
  10. Antitumor activity of an oncolytic measles virus against canine urinary bladder transitional cell carcinoma cells
    Authors: K Iizuka, K Shoji, T Fujiyuki, K Moritoh, K Tamura, A Yoshida, H Sato, M Yoneda, K Asano, C Kai
    Research in Veterinary Science, 2020-10-22;0(0):.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC
  11. Over-expression of Nectin-4 promotes progression of esophageal cancer and correlates with poor prognosis of the patients
    Authors: H Deng, H Shi, L Chen, Y Zhou, J Jiang
    Cancer Cell Int., 2019-04-23;19(0):106.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
    Authors: W Pratakpiri, AP Ping Teh, A Radtanakat, N Pirarat, N Thi Lan, M Takeda, S Techangams, R Yamaguchi
    Sci Rep, 2017-03-23;7(1):349.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development
    Authors: M Kitayama, K Mizutani, M Maruoka, K Mandai, S Sakakibara, Y Ueda, T Komori, Y Shimono, Y Takai
    J. Biol. Chem, 2016-01-12;291(11):5817-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer.
    Authors: Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y
    J Exp Clin Cancer Res, 2015-03-28;34(0):30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes.
    Authors: Alves L, Khosravi M, Avila M, Ader-Ebert N, Bringolf F, Zurbriggen A, Vandevelde M, Plattet P
    J Virol, 2015-03-18;89(10):5724-33.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC
  16. Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer.
    Authors: Galanis E, Atherton P, Maurer M, Knutson K, Dowdy S, Cliby W, Haluska P, Long H, Oberg A, Aderca I, Block M, Bakkum-Gamez J, Federspiel M, Russell S, Kalli K, Keeney G, Peng K, Hartmann L
    Cancer Res, 2014-11-14;75(1):22-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Nectin-4 mutations causing ectodermal dysplasia with syndactyly perturb the rac1 pathway and the kinetics of adherens junction formation.
    Authors: Fortugno P, Josselin E, Tsiakas K, Agolini E, Cestra G, Teson M, Santer R, Castiglia D, Novelli G, Dallapiccola B, Kurth I, Lopez M, Zambruno G, Brancati F
    J Invest Dermatol, 2014-02-27;134(8):2146-53.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  18. A role for PVRL4-driven cell-cell interactions in tumorigenesis.
    Authors: Pavlova N, Pallasch C, Elia A, Braun C, Westbrook T, Hemann M, Elledge S
    Elife, 2013-04-30;2(0):e00358.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Functional Assay, IHC, Western Blot
  19. Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008.
    J Virol, 2012-11-07;87(2):1105-14.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
    Authors: Noyce RS, Bondre DG, Ha MN
    PLoS Pathog., 2011-08-25;7(8):e1002240.
    Species: Hamster, Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  21. Development of Nectin4/FAP-targeted CAR-T cells secreting IL-7, CCL19, and IL-12 for malignant solid tumors
    Authors: Fanfan Li, Shuping Zhao, Cheng Wei, Yaodi Hu, Tianlong Xu, Xueyi Xin et al.
    Frontiers in Immunology
  22. Anticancer immunity induced by a synthetic tumor-targeted CD137 agonist
    Authors: Punit Upadhyaya, Johanna Lahdenranta, Kristen Hurov, Sailaja Battula, Rachel Dods, Eric Haines et al.
    Journal for ImmunoTherapy of Cancer

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