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Human NEDD8 Polyclonal Ab

Catalog #: A-812 Datasheet / COA / SDS
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Human NEDD8 Polyclonal Ab Summary

Species Reactivity
Human
Specificity
Antibody does not cross-react with ubiquitn, SUMO, or ISG15 proteins.  Non-functional for immunoprecipitation.
Source
Polyclonal Rabbit IgG
Immunogen
Full-length, highly purified human NEDD8 protein
Formulation
Boston Bio will fill in the Formulation info

Applications

Recommended Concentration
Sample
Western Blot
Recommended dilution range forWestern blot analysis is 1:100 to 1:1000.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Background: NEDD8

The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (APP-B1/UBA3), the Ubc12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase.  NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family. 

References
  1. Gong L. et al. (1999) J. Biol. Chem. 274:12036-12042
  2. Hori T. et al. (1999) Oncogene 18:6829-6834
  3. Kamura T. et al. (1999) Genes. Dev. 13:2928-2933
  4. Kumar S. et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
  5. Morimoto M. et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
  6. Wada H. et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
  7. Whitby F. G. et al. (1998) J. Biol. Chem. 273:34983-34991
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Entrez Gene IDs
4738 (Human); 18002 (Mouse); 25490 (Rat)
Alternate Names
FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8

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