Human Nestin Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Nestin in Human Neural Progenitor Cells. Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Nestin in A172 cells by Flow Cytometry. A172 cells were stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of Mouse Nestin by Western Blot Cardiolipin is externalized to the mitochondrial surface in alpha -syn mutant hNs and transgenic mice in response to alpha -syn accumulation. a Western analysis of lysates from corrected and A53T cells at DIV 14 and DIV 60 labeled for total alpha -syn, PS129, TH (DA neuronal marker), nestin (NPC marker) or GAPDH show that levels of PS129-modified alpha -syn are elevated in A53T hNs at DIV 14 and remain elevated at DIV 60 relative to corrected cells. b, c Mitochondria were purified from A53T cells and genetically corrected cells at both DIV 14 and DIV 60. Lysates were then separated based on TX-100 solubility (soluble) or urea solubility (insoluble). Western analysis of A53T cells shows elevated soluble alpha -syn at the mitochondria relative to corrected cells at DIV 14 and DIV 60 (denoted by ** and * respectively). Quantification of soluble and insoluble alpha -syn levels in mitochondrial fractions normalized to Coomassie (c). Data represent mean ± s.e.m. *P < 0.05, **P < 0.01 by ANOVA followed by Tukey’s post hoc test, n = 4. d, e Micrographs of corrected and A53T-mutant NPCs expressing the negative charge probe RPRE-RFP and CL-GFP show RPRE-RFP translocation from a primarily plasma membrane localization in isogenic-corrected hNs to a punctate intracellular localization in A53T hNs in tight cellular localization with CL-GFP (d). Quantification of percent total cell number with colocalized RPRE-RFP and CL-GFP (e). Data represent mean ± s.e.m. **P < 0.0001 by t-test, n = 3 coverslips over 2 independent differentiations, DIV: 14; scale bar: 10 μm. f, g Fluorescence resonance energy transfer (FRET) from CL-GFP to RPRE-RFP in hiPSC-derived A53T and corrected hNs or hESC-derived WT, A53T and E46K hNs was assessed (f) and mean FRET intensity was quantified (g). Data represent mean ± s.e.m. **P < 0.01 by ANOVA followed by Tukey’s post hoc test, for hiPSCs, n = 6, for hESCs, n = 8 coverslips over two independent differentiations, DIV: 14. Scale bar: 10 µm. h Mitochondria were isolated from the SNpc of 6-month-old WT and A53T transgenic animals and labeled with Annexin V to measure the abundance of cardiolipin on the mitochondrial surface. Controls were rotenone-exposed WT samples. Animals were not randomized, nor was the analysis blinded. Data represent mean ± s.e.m. **P < 0.01 by ANOVA followed by Dunnett post hoc test, n = 4. Clipart was obtained at clker.com Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03241-9), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Nestin by Immunohistochemistry MET and TMZ effects in in vivo glioma models(A) Variation of U251 tumor volume measured at contrast-enhanced T1 MRI after treatment expressed as percentage of volume change. Error bars indicate SEM. (B) U251 derived orthotopic brain tumors from vehicle (CTRL), TMZ or TMZ/metformin (Combo) treated mice were analyzed for Ki67, Nestin and CD133 markers by immunohistochemistry. No difference between the tumor edge and bulk areas in IHC staining intensities or percentages of positive cells was observed for any of the investigated markers. H&E was performed for morphological evaluation of the tumors. Representative images are shown with original magnification x200. (C) Variation of T98G tumor volume measured using caliper after treatment expressed as percentage of volume variation. In T98G xenograft model already at 7 days, the combined treatment significantly slowed down tumor growth compared to control (p value = 0.005), moreover a transient reduction was observed with MET (p value = 0.03). After 3 weeks, only Combo-treated mice displayed tumor smaller than vehicle (p value = 0.04). Error bars indicate SEM. (D) T98G tumors from vehicle (CTRL), TMZ, MET or TMZ/metformin (Combo) treated mice, were analyzed for Ki67, Nestin and CD133 markers expression by immunohistochemistry. No difference between the tumor edge and bulk areas in IHC staining intensities or percentages of positive cells was observed for any of the investigated markers. Hematoxylin and eosin staining (H&E) was performed for morphological evaluation of the tumors. Representative images are shown with Original magnification x200. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.23028), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Nestin by Immunohistochemistry MET and TMZ effects in in vivo glioma models(A) Variation of U251 tumor volume measured at contrast-enhanced T1 MRI after treatment expressed as percentage of volume change. Error bars indicate SEM. (B) U251 derived orthotopic brain tumors from vehicle (CTRL), TMZ or TMZ/metformin (Combo) treated mice were analyzed for Ki67, Nestin and CD133 markers by immunohistochemistry. No difference between the tumor edge and bulk areas in IHC staining intensities or percentages of positive cells was observed for any of the investigated markers. H&E was performed for morphological evaluation of the tumors. Representative images are shown with original magnification x200. (C) Variation of T98G tumor volume measured using caliper after treatment expressed as percentage of volume variation. In T98G xenograft model already at 7 days, the combined treatment significantly slowed down tumor growth compared to control (p value = 0.005), moreover a transient reduction was observed with MET (p value = 0.03). After 3 weeks, only Combo-treated mice displayed tumor smaller than vehicle (p value = 0.04). Error bars indicate SEM. (D) T98G tumors from vehicle (CTRL), TMZ, MET or TMZ/metformin (Combo) treated mice, were analyzed for Ki67, Nestin and CD133 markers expression by immunohistochemistry. No difference between the tumor edge and bulk areas in IHC staining intensities or percentages of positive cells was observed for any of the investigated markers. Hematoxylin and eosin staining (H&E) was performed for morphological evaluation of the tumors. Representative images are shown with Original magnification x200. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.23028), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Nestin by Immunocytochemistry/Immunofluorescence Neural stem cells could proliferate and differentiate into neurons and astrocytes.(A) Representative photomicrograph of neurospheres in culture. (B) Immunocytochemical staining of purified NSCs with Nestin. (C) Immunocytochemical staining of purified protoplasmic astrocytes with GFAP. (D) Immunocytochemical staining of purified neurons with beta -tubulin-III. (E) Nucleus staining of differentiated cells from NSCs with DAPI. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26431046), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Nestin by Western Blot miR–381 promoted neural stem cells proliferation.(A) The expression of miR–381 was measured by qRT-PCR. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of nestin was detected by qRT-PCR. (D) The protein expression of nestin was measured by Western blot. **p<0.01 and ***p<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26431046), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Nestin by Immunohistochemistry Dissimilar tumorigenesis in the in vivo animal model.(a) Kaplan-Meier survival plots; 3 mice were used for cells of each clone. (b) Representative brain tumors of NOD-SCID mice harboring xenografts of the clones #2 (upper left), #3 (lower left), #4 (upper right) and #5 (lower right); H.E. staining, arrows: tumor, scale bar = 5 mm. (c) A representative xenograft of the clone #4; nestin-positive cells infiltrated the contralateral hemisphere (arrows). Scale bars = 5 mm (left), 1 mm (middle), 50 μm (right). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25623281), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human Nestin Antibody by Western Blot Cardiolipin is externalized to the mitochondrial surface in alpha -syn mutant hNs and transgenic mice in response to alpha -syn accumulation. a Western analysis of lysates from corrected and A53T cells at DIV 14 and DIV 60 labeled for total alpha -syn, PS129, TH (DA neuronal marker), nestin (NPC marker) or GAPDH show that levels of PS129-modified alpha -syn are elevated in A53T hNs at DIV 14 and remain elevated at DIV 60 relative to corrected cells. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29483518), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nestin
Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
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Product Datasheets
Citations for Human Nestin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
161
Citations: Showing 1 - 10
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PDGF-AA and BDNF Promote Neural Stem Cell Differentiation.
Authors: Soltys J, Perrone C, Knight J, Mao-Draayer Y.
Lab Chip
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Loss of Protein Function Causing Severe Phenotypes of Female-Restricted Wieacker Wolff Syndrome due to a Novel Nonsense Mutation in the ZC4H2 Gene
Authors: Jing-Jing Sun, Qin Cai, Miao Xu, Yan-Na Liu, Wan-Rui Li, Juan Li et al.
Genes (Basel)
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Single-cell RNA sequencing of neural stem cells derived from human trisomic iPSCs reveals the abnormalities during neural differentiation of Down syndrome
Authors: Jia-jun Qiu, Yan-na Liu, Hao Wei, Fanyi Zeng, Jing-bin Yan
Frontiers in Molecular Neuroscience
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NF-?B Activity Initiates Human ESC-Derived Neural Progenitor Cell Differentiation by Inducing a Metabolic Maturation Program
Authors: LM FitzPatric, KE Hawkins, JMKM Delhove, E Fernandez, C Soldati, LF Bullen, A Nohturfft, SN Waddington, DL Medina, JP Bolaños, TR McKay
Stem Cell Reports, 2018-04-19;0(0):.
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Dopaminergic progenitors generated by small molecule approach survived, integrated, and promoted functional recovery in (6-OHDA) mouse model of Parkinson's disease
Authors: Alexanian, AR;Sorokin, A;Duersteler, M;
Journal of the neurological sciences
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Atypical cellular responses mediated by intracellular constitutive active TrkB (NTRK2) kinase domains and a solely intracellular NTRK2-fusion oncogene
Authors: Gupta, R;Dittmeier, M;Wohlleben, G;Nickl, V;Bischler, T;Luzak, V;Wegat, V;Doll, D;Sodmann, A;Bady, E;Langlhofer, G;Wachter, B;Havlicek, S;Gupta, J;Horn, E;Lüningschrör, P;Villmann, C;Polat, B;Wischhusen, J;Monoranu, CM;Kuper, J;Blum, R;
Cancer gene therapy
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
CRISPR/Cas9-based GLA knockout to generate the female Fabry disease human induced pluripotent stem cell line MHHi001-A-15
Authors: Juchem, M;Lehmann, N;Behrens, YL;Bär, C;Thum, T;Hoepfner, J;
Stem cell research
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of human induced pluripotent stem cell line MHHi029-A from a male Fabry disease patient carrying c.959A > T mutation
Authors: Jahn, C;Juchem, M;Sonnenschein, K;Gietz, A;Buchegger, T;Lachmann, N;Göhring, G;Behrens, YL;Bär, C;Thum, T;Hoepfner, J;
Stem cell research
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Immunocytochemistry -
Generation of complex bone marrow organoids from human induced pluripotent stem cells
Authors: Frenz-Wiessner, S;Fairley, SD;Buser, M;Goek, I;Salewskij, K;Jonsson, G;Illig, D;Zu Putlitz, B;Petersheim, D;Li, Y;Chen, PH;Kalauz, M;Conca, R;Sterr, M;Geuder, J;Mizoguchi, Y;Megens, RTA;Linder, MI;Kotlarz, D;Rudelius, M;Penninger, JM;Marr, C;Klein, C;
Nature methods
Species: Human
Sample Types: Organoid
Applications: Immunohistochemistry -
Selective gene expression maintains human tRNA anticodon pools during differentiation
Authors: Gao, L;Behrens, A;Rodschinka, G;Forcelloni, S;Wani, S;Strasser, K;Nedialkova, DD;
Nature cell biology
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Nuclear Glycoprotein A Repetitions Predominant (GARP) Is a Common Trait of Glioblastoma Stem-like Cells and Correlates with Poor Survival in Glioblastoma Patients
Authors: Zimmer, N;Trzeciak, ER;Müller, A;Licht, P;Sprang, B;Leukel, P;Mailänder, V;Sommer, C;Ringel, F;Tuettenberg, J;Kim, E;Tuettenberg, A;
Cancers
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC -
Comparison of two protocols for the generation of iPSC-derived human astrocytes
Authors: Patrycja Mulica, Carmen Venegas, Zied Landoulsi, Katja Badanjak, Sylvie Delcambre, Maria Tziortziou et al.
Biol Proced Online
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Production of Blended Poly(acrylonitrile): Poly(ethylenedioxythiophene):Poly(styrene sulfonate) Electrospun Fibers for Neural Applications
Authors: Garrudo, FFF;Filippone, G;Resina, L;Silva, JC;Barbosa, F;Ferreira, LFV;Esteves, T;Marques, AC;Morgado, J;Ferreira, FC;
Polymers
Species: Human
Sample Types: Whole Cells
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Microcephaly-associated protein WDR62 shuttles from the Golgi apparatus to the spindle poles in human neural progenitors
Authors: Dell'Amico, C;Angulo Salavarria, MM;Takeo, Y;Saotome, I;Dell'Anno, MT;Galimberti, M;Pellegrino, E;Cattaneo, E;Louvi, A;Onorati, M;
eLife
Species: Human
Sample Types: Whole Cells
Applications: ICC -
IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries
Authors: Tam, KW;Wong, CY;Wu, KL;Lam, G;Liang, X;Wong, WT;Li, MT;Liu, WY;Cai, S;Shea, GK;Shum, DK;Chan, YS;
Cells
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis
Authors: Karapurkar JK, Kim MS, Colaco JC et al.
Journal of experimental & clinical cancer research : CR
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A simplified protocol for the generation of cortical brain organoids
Authors: KN Eigenhuis, HB Somsen, M van der Kr, H Smeenk, AL Korporaal, SA Kushner, FMS de Vrij, DLC van den Be
Frontiers in Cellular Neuroscience, 2023-04-04;17(0):1114420.
Species: Human
Sample Types: Organoid
Applications: ICC -
BCL7A-containing SWI/SNF/BAF complexes modulate mitochondrial bioenergetics during neural progenitor differentiation
Authors: Wischhof L, Lee HM, Tutas J et al.
The EMBO Journal
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The oncogenic JAG1 intracellular domain is a transcriptional cofactor that acts in concert with DDX17/SMAD3/TGIF2
Authors: EJ Kim, JY Kim, SO Kim, N Hong, SH Choi, MG Park, J Jang, SW Ham, S Seo, SY Lee, K Lee, HJ Jeong, SJ Kim, S Jeong, K Min, SC Kim, X Jin, SH Kim, SH Kim, H Kim
Cell Reports, 2022-11-22;41(8):111626.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Vestibular Nuclei: A New Neural Stem Cell Niche?
Authors: G Rastoldo, I Watabe, A Lapotre, A Tonetto, A López-Juár, B Tighilet
Cells, 2022-11-14;11(22):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells
Authors: L Adnani, J Kassouf, B Meehan, C Spinelli, N Tawil, I Nakano, J Rak
Nature Communications, 2022-09-19;13(1):5494.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases
Authors: J Brás, D Henriques, R Moreira, MM Santana, R Silva-Pedr, D Adão, S Braz, AR Álvaro, LP de Almeida, LS Mendonça
Scientific Reports, 2022-07-22;12(1):12513.
Species: Human
Sample Types: Organoids
Applications: IHC -
Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
International Journal of Molecular Sciences, 2022-06-30;23(13):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement
Authors: Elena Fdez, Jesús Madero-Pérez, Antonio J. Lara Ordóñez, Yahaira Naaldijk, Rachel Fasiczka, Ana Aiastui et al.
iScience
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Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1
Authors: YH Chen, PS Huang, MH Wen, M Pan, DF Lee, TY Chen
Oncogene, 2022-06-14;63(0):102845.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
Authors: Aida Amador-Álvarez, María A. Gómez-Muñoz, Ismael Rodríguez-Prieto, Ricardo Pardal, Francisco M. Vega
STAR Protocols
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Centrin 2: A Novel Marker of Mature and Neoplastic Human Astrocytes
Authors: Elisa Degl’Innocenti, Tino Emanuele Poloni, Valentina Medici, Luca Recupero, Claudia Dell’Amico, Eleonora Vannini et al.
Frontiers in Cellular Neuroscience
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Single metal-organic framework-embedded nanopit arrays: A new way to control neural stem cell differentiation
Authors: YW Cho, S Jee, IR Suhito, JH Lee, CG Park, KM Choi, TH Kim
Science Advances, 2022-04-20;8(16):eabj7736.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ICC -
Generation of the human induced pluripotent stem cell line (IBKMOLi002-A) from PBMCs of a patient carrying the heterozygous L271H mutation of the voltage-gated calcium channel subunit Cav1.3-encoding CACNA1D gene
Authors: M Tisch, M Carmen De, M Suarez-Cub, C Fauth, M Defrancesc, J Zschocke, K Günther, F Edenhofer
Stem Cell Research, 2022-04-09;61(0):102784.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Steady-State Levels of Miro1 Linked to Phosphorylation at Serine 156 and Mitochondrial Respiration in Dopaminergic Neurons
Authors: L Schwarz, JC Fitzgerald
Cells, 2022-04-08;11(8):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis
Authors: K Kaushal, EJ Kim, A Tyagi, JK Karapurkar, S Haq, HS Jung, KS Kim, S Ramakrishn
Cell Death and Differentiation, 2022-03-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Differences in stem cell marker and osteopontin expression in primary and recurrent glioblastoma
Authors: B Polat, G Wohlleben, R Kosmala, D Lisowski, F Mantel, V Lewitzki, M Löhr, R Blum, P Herud, M Flentje, CM Monoranu
Cancer Cell International, 2022-02-19;22(1):87.
Species: Human
Sample Types: Whole Tissue
Applications: IF, IHC -
Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells
Authors: B Bohl, A Jabali, J Ladewig, P Koch
Science Advances, 2022-02-11;8(6):eabl5792.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human neural cell type-specific�extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain
Authors: You Y, Muraoka S, Jedrychowski MP et al.
Journal of Extracellular Vesicles
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Profiling Glioblastoma Cases with an Expression of DCX, OLIG2 and NES
Authors: Adrian Odrzywolski, Bożena Jarosz, Michał Kiełbus, Ilona Telejko, Dominik Ziemianek, Sebastian Knaga et al.
International Journal of Molecular Sciences
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Gene-corrected p.A30P SNCA patient-derived isogenic neurons rescue neuronal branching and function
Authors: PA Barbuti, J Ohnmacht, BFR Santos, PM Antony, F Massart, G Cruciani, CM Dording, L Pavelka, N Casadei, YJ Kwon, R Krüger
Scientific Reports, 2021-11-09;11(1):21946.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development
Authors: Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen
Stem Cell Research, 2021-10-22;57(0):102583.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Viral targeting of glioblastoma stem cells with patient-specific genetic and post-translational p53 deregulations
Authors: J Gil-Ranedo, C Gallego-Ga, JM Almendral
Cell Reports, 2021-09-07;36(10):109673.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Rapid target validation in a Cas9-inducible hiPSC derived kidney model
Authors: Y Shamshirga, A Jonebring, A Svensson, I Leefa, M Bohlooly-Y, M Firth, KJ Woollard, A Hofherr, IM Rogers, R Hicks
Scientific Reports, 2021-08-16;11(1):16532.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells
Authors: S Rajasingh, V Sigamani, V Selvam, N Gurusamy, S Kirankumar, J Vasanthan, J Rajasingh
Journal of Cellular and Molecular Medicine, 2021-08-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability
Authors: F Xiong, R Wang, JH Lee, S Li, SF Chen, Z Liao, LA Hasani, PT Nguyen, X Zhu, J Krakowiak, DF Lee, L Han, KL Tsai, Y Liu, W Li
Cell Research, 2021-06-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution
Authors: Fabio Bertan, Lena Wischhof, Enzo Scifo, Mihaela Guranda, Joshua Jackson, Anaïs Marsal-Cots et al.
Cell Reports Methods
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Restoring miR-132 expression rescues adult hippocampal neurogenesis and memory deficits in Alzheimer's disease
Authors: H Walgrave, S Balusu, S Snoeck, E Vanden Eyn, K Craessaert, N Thrupp, L Wolfs, K Horré, Y Fourne, A Ronisz, E Silajdži?, A Penning, G Tosoni, Z Callaerts-, R D'Hooge, DR Thal, H Zetterberg, S Thuret, M Fiers, CS Frigerio, B De Stroope, E Salta
Cell Stem Cell, 2021-05-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Decidualization modulates the mesenchymal stromal/stem cell and pericyte characteristics of human decidual stromal cells. Effects on antigen expression, chemotactic activity on monocytes and antitumoral activity
Authors: MJ Ruiz-Magañ, R Martinez-A, T Llorca, AC Abadia-Mol, C Ruiz-Ruiz, EG Olivares
Journal of reproductive immunology, 2021-05-01;145(0):103326.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants
Authors: RB Bressan, B Southgate, KM Ferguson, C Blin, V Grant, N Alfazema, JC Wills, MA Marques-To, GM Morrison, J Ashmore, F Robertson, CAC Williams, L Bradley, A von Kriegs, RA Anderson, SR Tomlinson, SM Pollard
Cell Stem Cell, 2021-02-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus
Authors: Elena Morelli, Elisa A. Speranza, Enrica Pellegrino, Galina V. Beznoussenko, Francesca Carminati, Massimiliano Garré et al.
Frontiers in Cell and Developmental Biology
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Cytoskeletal Proteins in Myotendinous Junctions of Human Extraocular Muscles
Authors: Jing-Xia Liu, Fatima Pedrosa Domellöf
Investigative Opthalmology & Visual Science
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Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs)
Authors: Ryutaro Kajihara, Tadahiro Numakawa, Takumi Era
BIO-PROTOCOL
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Activity-dependent regulome of human GABAergic neurons reveals new patterns of gene regulation and neurological disease heritability
Authors: GL Boulting, E Durresi, B Ataman, MA Sherman, K Mei, DA Harmin, AC Carter, DR Hochbaum, AJ Granger, JM Engreitz, S Hrvatin, MR Blanchard, MG Yang, EC Griffith, ME Greenberg
Nature Neuroscience, 2021-02-04;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix
Authors: M Matoug-Elw, H Nazzal, EM Raif, SP Wilshaw, F Esteves, M Duggal
Scientific Reports, 2020-12-09;10(1):21553.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
DNA methylation changes in Down syndrome derived neural iPSCs uncover co-dysregulation of ZNF and HOX3 families of transcription factors
Authors: Loora Laan, Joakim Klar, Maria Sobol, Jan Hoeber, Mansoureh Shahsavani, Malin Kele et al.
Clinical Epigenetics
-
Identification of VRK1 as a New Neuroblastoma Tumor Progression Marker Regulating Cell Proliferation
Authors: A Colmenero-, MA Gómez-Muño, I Rodríguez-, A Amador-Álv, KO Henrich, D Pascual-Va, K Okonechnik, E Rivas, F Westermann, R Pardal, FM Vega
Cancers (Basel), 2020-11-20;12(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The HIF1&alpha/JMY pathway promotes glioblastoma stem-like cell invasiveness after irradiation
Authors: LR Gauthier, M Saati, H Bensalah-P, K Ben M'Bare, O Gitton-Que, R Bertrand, D Busso, MA Mouthon, A Collura, MP Junier, H Chneiweiss, JR Pineda, FD Boussin
Sci Rep, 2020-10-30;10(1):18742.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Generation of a human iPSC line CIBi008-A from amniotic fluid-derived cells of a fetus with &beta-thalassemia carrying variants of -28A�>�G and IVS-II-654C�>�T in HBB
Authors: S Wu, H Wei, M Chu, Z Weng
Stem Cell Res, 2020-10-29;49(0):102074.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Modeling Klinefelter Syndrome Using Induced Pluripotent Stem Cells Reveals Impaired Germ Cell Differentiation
Authors: Olivier Botman, Youssef Hibaoui, Maria G. Giudice, Jérôme Ambroise, Catherine Creppe, Anis Feki et al.
Frontiers in Cell and Developmental Biology
-
Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson's Disease Point Mutation A30P
Authors: P Barbuti, P Antony, B Santos, F Massart, G Cruciani, C Dording, J Arias, J Schwamborn, R Krüger
Cells, 2020-09-10;9(9):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
EGFR/FOXO3a/BIM signaling pathway determines chemosensitivity of BMP4-differentiated glioma stem cells to temozolomide
Authors: IA Ciechomska, B Gielniewsk, B Wojtas, B Kaminska, J Mieczkowsk
Exp. Mol. Med., 2020-08-12;0(0):.
Species: Human
Sample Types: Cell Lysates, Protein
Applications: Western Blot -
Effect of amniotic fluid stem cell transplantation on the recovery of bladder dysfunction in spinal cord-injured rats
Authors: CC Liang, SS Shaw, YS Ko, YH Huang, TH Lee
Sci Rep, 2020-06-22;10(1):10030.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Development of immortalized human hepatocyte-like hybrid cells by fusion of multi-lineage progenitor cells with primary hepatocytes
Authors: DP Collins, JH Hapke, RN Aravalli, CJ Steer
PLoS ONE, 2020-06-04;15(6):e0234002.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Impaired mitochondrial–endoplasmic reticulum interaction and mitophagy in Miro1-mutant neurons in Parkinson’s disease
Authors: Clara Berenguer-Escuder, Dajana Grossmann, Paul Antony, Giuseppe Arena, Kobi Wasner, François Massart et al.
Human Molecular Genetics
-
Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
Cell Rep, 2020-05-05;31(5):107599.
Species: Human, Mouse
Sample Types:
Applications: Cell Culture, IF -
Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke
Authors: SH Oh, YW Jeong, W Choi, JE Noh, S Lee, HS Kim, J Song
Stem Cells Int, 2020-03-23;2020(0):4061516.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
FKBP9 promotes the malignant behavior of glioblastoma cells and confers resistance to endoplasmic reticulum stress inducers
Authors: H Xu, P Liu, Y Yan, K Fang, D Liang, X Hou, X Zhang, S Wu, J Ma, R Wang, T Li, H Piao, S Meng
J. Exp. Clin. Cancer Res., 2020-02-28;39(1):44.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Long-Term Stability and Differentiation Potential of Cryopreserved cGMP-Compliant Human Induced Pluripotent Stem Cells
Authors: Mehdi Shafa, Tylor Walsh, Krishna Morgan Panchalingam, Thomas Richardson, Laura Menendez, Xinghui Tian et al.
International Journal of Molecular Sciences
-
End-to-End Platform for Human Pluripotent Stem Cell Manufacturing
Authors: Puspa R. Pandey, Amarel Tomney, Marites T. Woon, Nicholas Uth, Farjad Shafighi, Igor Ngabo et al.
International Journal of Molecular Sciences
-
IQGAP1, AmotL2, and FKBP51 Scaffoldins in the Glioblastoma Microenvironment
Authors: Deborah Rotoli, Manuel Morales, María-del-C. Maeso, Julio Ávila, Natalia D. Pérez-Rodríguez, Ali Mobasheri et al.
Journal of Histochemistry & Cytochemistry
-
Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis
Authors: M Huang, J Tailor, Q Zhen, AH Gillmor, ML Miller, H Weishaupt, J Chen, T Zheng, EK Nash, LK McHenry, Z An, F Ye, Y Takashima, J Clarke, H Ayetey, FMG Cavalli, B Luu, BS Moriarity, S Ilkhanizad, L Chavez, C Yu, KM Kurian, T Magnaldo, N Sevenet, P Koch, SM Pollard, P Dirks, MP Snyder, DA Largaespad, YJ Cho, JJ Phillips, FJ Swartling, AS Morrissy, M Kool, SM Pfister, MD Taylor, A Smith, WA Weiss
Cell Stem Cell, 2019-06-13;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity
Authors: S Chanda, CE Ang, QY Lee, M Ghebrial, D Haag, Y Shibuya, M Wernig, TC Südhof
Cell Stem Cell, 2019-05-30;25(1):103-119.e6.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Transplantation of clinical-grade human neural stem cells reduces neuroinflammation, prolongs survival and delays disease progression in the SOD1 rats
Authors: C Zalfa, L Rota Nodar, E Vacchi, M Gelati, D Profico, M Boido, E Binda, L De Filippi, M Copetti, V Garlatti, P Daniele, J Rosati, A De Luca, F Pinos, L Cajola, A Visioli, L Mazzini, A Vercelli, M Svelto, AL Vescovi, D Ferrari
Cell Death Dis, 2019-04-25;10(5):345.
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC -
Characterization of the first induced pluripotent stem cell line generated from a patient with autosomal dominant catecholaminergic polymorphic ventricular tachycardia due to a heterozygous mutation in cardiac calsequestrin-2
Authors: S Ross, M Holliday, S Lim, C Semsarian
Stem Cell Res, 2019-04-25;37(0):101450.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain
Authors: J Luzuriaga, O Pastor-Alo, JM Encinas, F Unda, G Ibarretxe, JR Pineda
Front Physiol, 2019-03-28;10(0):347.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
Authors: BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd
Sci Rep, 2019-03-20;9(1):4902.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes
Authors: I Bertolini, A Terrasi, C Martelli, G Gaudioso, A Di Cristof, AM Storaci, M Formica, P Braidotti, K Todoerti, S Ferrero, M Caroli, L Ottobrini, T Vaccari, V Vaira
EBioMedicine, 2019-02-06;0(0):.
Species: Human
Sample Types: Tissue Array
Applications: IHC -
The novel lncRNA lnc-NR2F1 is pro-neurogenic and mutated in human neurodevelopmental disorders
Authors: Cheen Euong Ang, Qing Ma, Orly L Wapinski, ShengHua Fan, Ryan A Flynn, Qian Yi Lee et al.
eLife
-
Expression and secretion of synaptic proteins during stem cell differentiation to cortical neurons
Authors: Faisal Hayat Nazir, Bruno Becker, Ann Brinkmalm, Kina Höglund, Åsa Sandelius, Petra Bergström et al.
Neurochemistry International
-
Aberrant neuronal differentiation is common in glioma but is associated neither with epileptic seizures nor with better survival
Authors: CP Beier, T Rasmussen, RH Dahlrot, HB Tenstad, JS Aarø, MF Sørensen, SB Heimisdótt, MD Sørensen, P Svenningse, MJ Riemenschn, D Beier, BW Kristensen
Sci Rep, 2018-10-08;8(1):14965.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of an induced pluripotent stem cell line from a hypertrophic cardiomyopathy patient with a pathogenic myosin binding protein C (MYBPC3) p.Arg502Trp mutation
Authors: M Holliday, SB Ross, S Lim, C Semsarian
Stem Cell Res, 2018-10-06;33(0):56-59.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
An RGD small-molecule integrin antagonist induces detachment-mediated anoikis in glioma cancer stem cells
Authors: Mayra Paolillo, Marisa C Galiazzo, Antonio Daga, Emilio Ciusani, Massimo Serra, Lino Colombo et al.
International Journal of Oncology
-
Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies
Authors: J Rosati, D Ferrari, F Altieri, S Tardivo, C Ricciolini, C Fusilli, C Zalfa, DC Profico, F Pinos, L Bernardini, B Torres, I Manni, G Piaggio, E Binda, M Copetti, G Lamorte, T Mazza, M Carella, M Gelati, EM Valente, A Simeone, AL Vescovi
Cell Death Dis, 2018-09-17;9(10):937.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
GMP Production and Scale-Up of Adherent Neural Stem Cells with a Quantum Cell Expansion System
Authors: R Tirughana, MZ Metz, Z Li, C Hall, D Hsu, J Beltzer, AJ Annala, D Oganesyan, M Gutova, KS Aboody
Mol Ther Methods Clin Dev, 2018-07-07;10(0):48-56.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A comparative study of the neural stem cell niche in the adult hypothalamus of human, mouse, rat and gray mouse lemur (Microcebus murinus )
Authors: Giuliana Pellegrino, Claire Trubert, Jérémy Terrien, Fabien Pifferi, Danièle Leroy, Anne Loyens et al.
Journal of Comparative Neurology
-
Selective pharmacological inhibition of DDR1 prevents experimentally-induced glomerulonephritis in prevention and therapeutic regime
Authors: S Moll, Y Yasui, A Abed, T Murata, H Shimada, A Maeda, N Fukushima, M Kanamori, S Uhles, L Badi, T Cagarelli, I Formentini, F Drawnel, G Georges, T Bergauer, R Gasser, RD Bonfil, R Fridman, H Richter, J Funk, MJ Moeller, C Chatzianto, M Prunotto
J Transl Med, 2018-06-01;16(1):148.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Establishment of Insulin-Producing Cells From Human Embryonic Stem Cells Underhypoxic Condition for Cell Based Therapy
Authors: Piyaporn Rattananinsruang, Chavaboon Dechsukhum, Wilairat Leeanansaksiri
Frontiers in Cell and Developmental Biology
-
Genome Editing in Neuroepithelial Stem Cells to Generate Human Neurons with High Adenosine-Releasing Capacity
Authors: D Poppe, J Doerr, M Schneider, R Wilkens, JA Steinbeck, J Ladewig, A Tam, DE Paschon, PD Gregory, A Reik, CE Müller, P Koch, O Brüstle
Stem Cells Transl Med, 2018-03-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers
Authors: M Shafa, F Yang, T Fellner, MS Rao, BA Baghbadera
Front Med (Lausanne), 2018-03-15;5(0):69.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease
Authors: L Marrone, C Bus, D Schöndorf, JC Fitzgerald, M Kübler, B Schmid, P Reinhardt, L Reinhardt, M Deleidi, T Levin, A Meixner, B Klink, M Glatza, CJ Gloeckner, T Gasser, J Sternecker
PLoS ONE, 2018-03-07;13(3):e0192497.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria
Authors: F Hsu, S Spannl, C Ferguson, AA Hyman, RG Parton, M Zerial
Elife, 2018-02-22;7(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Intracellular pH Regulation in iPSCs-derived Astrocytes from Subjects with Chronic Mountain Sickness
Authors: H Yao, H Zhao, J Wang, GG Haddad
Neuroscience, 2018-02-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Intrinsic Immunity Shapes Viral Resistance of Stem Cells
Authors: Xianfang Wu, Viet Loan Dao Dao Thi, Yumin Huang, Eva Billerbeck, Debjani Saha, Hans-Heinrich Hoffmann et al.
Cell
-
Metformin and temozolomide, a synergic option to overcome resistance in glioblastoma multiforme models
Authors: Silvia Valtorta, Alessia Lo Lo Dico, Isabella Raccagni, Daniela Gaglio, Sara Belloli, Letterio S. Politi et al.
Oncotarget
-
Easy xeno-free and feeder-free method for isolating and growing limbal stromal and epithelial stem cells of the human cornea
Authors: D Ghoubay-Be, C de Sousa, R Martos, G Latour, MC Schanne-Kl, E Dupin, V Borderie
PLoS ONE, 2017-11-17;12(11):e0188398.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways
Authors: F Wang, AY Wang, C Chesnelong, Y Yang, A Nabbi, S Thalappill, V Alekseev, K Riabowol
Oncogene, 2017-09-18;37(3):286-301.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value
Authors: AM Rosager, MD Sørensen, RH Dahlrot, S Hansen, DL Schonberg, JN Rich, JD Lathia, BW Kristensen
PLoS ONE, 2017-08-24;12(8):e0182954.
Species: Human
Sample Types: Whole Cells
Applications: IHC-P -
Induction of senescence in primary glioblastoma cells by serum and TGF?
Authors: R Kumar, A Gont, TJ Perkins, JEL Hanson, IAJ Lorimer
Sci Rep, 2017-05-19;7(1):2156.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
Authors: X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky
PLoS ONE, 2017-04-25;12(4):e0174074.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
Oncotarget, 2017-04-25;8(17):28074-28092.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
Cell Rep, 2017-04-04;19(1):20-35.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
Authors: SS Jensen, SA Petterson, B Halle, C Aaberg-Jes, BW Kristensen
BMC Cancer, 2017-03-07;17(1):178.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene
Authors: SB Ross, ST Fraser, N Nowak, C Semsarian
Stem Cell Res, 2017-03-07;20(0):88-90.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells
Authors: Raul Bardini Bressan, Pooran Singh Dewari, Maria Kalantzaki, Ester Gangoso, Mantas Matjusaitis, Claudia Garcia-Diaz et al.
Development
-
IQGAP1 in Podosomes/Invadosomes Is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status
Authors: Deborah Rotoli, Natalia Dolores Pérez-Rodríguez, Manuel Morales, María Del Carmen Maeso, Julio Ávila, Ali Mobasheri et al.
International Journal of Molecular Sciences
-
RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells
Authors: HO King, T Brend, HL Payne, A Wright, TA Ward, K Patel, T Egnuni, LF Stead, A Patel, H Wurdak, SC Short
Stem Cell Reports, 2017-01-10;8(1):125-139.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot -
Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation and�Motility
Stem Cell Reports, 2016-12-29;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors
PLoS ONE, 2016-12-28;11(12):e0169129.
Species: Human
Sample Types: Whole Tissue
Applications: ICC -
BIX01294, an inhibitor of histone methyltransferase, induces autophagy-dependent differentiation of glioma stem-like cells
Sci Rep, 2016-12-09;6(0):38723.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ICC, Western Blot -
Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization
Authors: Florian Wegner
J. Neurosci., 2016-11-23;36(47):12027-12043.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype
PLoS ONE, 2016-05-12;11(5):e0155106.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Alteration of DNA damage signaling pathway profile in radiation-treated glioblastoma stem-like cells
Authors: CHAO SUN, ZHONGYONG WANG, WUCHAO SONG, BAOMIN CHEN, JINSHI ZHANG, XINGLIANG DAI et al.
Oncology Letters
-
Characterization of bipolar disorder patient-specific induced pluripotent stem cells from a family reveals neurodevelopmental and mRNA expression abnormalities
Authors: Jon M. Madison, Fen Zhou, Aparna Nigam, Ali Hussain, Douglas D. Barker, Ralda Nehme et al.
Molecular Psychiatry
-
Genome-Wide Definition of Promoter and Enhancer Usage during Neural Induction of Human Embryonic Stem Cells
Authors: Valentina Poletti, Alessia Delli Carri, Guidantonio Malagoli Tagliazucchi, Andrea Faedo, Luca Petiti, Emilia Maria Cristina Mazza et al.
PLOS ONE
-
Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells.
Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M
Stem Cells, 2015-04-01;33(4):1142-52.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The evidence of glioblastoma heterogeneity.
Authors: Soeda, Akio, Hara, Akira, Kunisada, Takahiro, Yoshimura, Shin-ich, Iwama, Toru, Park, Deric M
Sci Rep, 2015-01-27;5(0):7979.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
A preclinical mouse model of glioma with an alternative mechanism of telomere maintenance (ALT).
Authors: Jeitany M, Pineda J, Liu Q, Porreca R, Hoffschir F, Desmaze C, Silvestre D, Mailliet P, Junier M, Londono-Vallejo A, Segal-Bendirdjian E, Chneiweiss H, Boussin F
Int J Cancer, 2014-09-11;136(7):1546-58.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice
Authors: Daniel L. Haus, Hal X. Nguyen, Eric M. Gold, Noriko Kamei, Harvey Perez, Harry D. Moore et al.
Stem Cell Research
-
Crosstalk between Glioma-Initiating Cells and Endothelial Cells Drives Tumor Progression
Authors: Hye-Min Jeon, Sung-Hak Kim, Xun Jin, Jong Bae Park, Se Hoon Kim, Kaushal Joshi et al.
Cancer Research
-
Molecular evidence for OCT4-induced plasticity in adult human fibroblasts required for direct cell fate conversion to lineage specific progenitors.
Authors: Mitchell R, Szabo E, Shapovalova Z, Aslostovar L, Makondo K, Bhatia M
Stem Cells, 2014-08-01;32(8):2178-87.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
CD133-positive cancer stem cells from colo205 human colon adenocarcinoma cell line show resistance to chemotherapy and display a specific metabolomic profile
Authors: Zangiacomi Vincent, Kenichi Urakami, Koji Maruyama, Ken Yamaguchi, Masatoshi Kusuhara
Genes & Cancer
-
Phenotypic plasticity in normal breast derived epithelial cells.
Authors: Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S
BMC Cell Biol, 2014-06-10;15(0):20.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Embryonic stem cell-derived neural progenitors as non-tumorigenic source for dopaminergic neurons
Authors: Mei-Chih Liao, Mihaela Diaconu, Sebastian Monecke, Patrick Collombat, Charles Timaeus, Tanja Kuhlmann et al.
World Journal of Stem Cells
-
Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma.
Authors: Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T
Int J Oncol, 2014-01-24;44(4):1118-30.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P -
Novel anti-glioblastoma agents and therapeutic combinations identified from a collection of FDA approved drugs.
Authors: Jiang, Pengfei, Mukthavaram, Rajesh, Chao, Ying, Bharati, Ila Sri, Fogal, Valentin, Pastorino, Sandra, Cong, Xiuli, Nomura, Natsuko, Gallagher, Matt, Abbasi, Taher, Vali, Shireen, Pingle, Sandeep, Makale, Milan, Kesari, Santosh
J Transl Med, 2014-01-17;12(0):13.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Epigenetic silencing of miRNA-9 is associated with HES1 oncogenic activity and poor prognosis of medulloblastoma.
Authors: Fiaschetti G, Abela L, Nonoguchi N, Dubuc A, Remke M, Boro A, Grunder E, Siler U, Ohgaki H, Taylor M, Baumgartner M, Shalaby T, Grotzer M
Br J Cancer, 2013-12-17;110(3):636-47.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
Functional Impacts of NRXN1 Knockdown on Neurodevelopment in Stem Cell Models
Authors: Liyun Zeng, Peilin Zhang, Lingling Shi, Vicky Yamamoto, Wange Lu, Kai Wang
PLoS ONE
-
Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
Authors: Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel C, Hoing S, Moritz S, Parga J, Wagner L, Bruder J, Wu G, Schmid B, Ropke A, Klingauf J, Schwamborn J, Gasser T, Scholer H, Sterneckert J
PLoS ONE, 2013-03-22;8(3):e59252.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Embryonic stem cell-based modeling of tau pathology in human neurons.
Authors: Mertens J, Stuber K, Poppe D, Doerr J, Ladewig J, Brustle O, Koch P
Am J Pathol, 2013-03-13;182(5):1769-79.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Activation of the type I interferon pathway is enhanced in response to human neuronal differentiation.
Authors: Farmer, Jocelyn, Altschaefl, Kate M, O'Shea, K Sue, Miller, David J
PLoS ONE, 2013-03-07;8(3):e58813.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Nestin is highly expressed in advanced-stage melanomas and neurotized nevi.
Authors: Akiyama, Michiko, Matsuda, Yoko, Ishiwata, Toshiyuk, Naito, Zenya, Kawana, Seiji
Oncol Rep, 2013-02-15;29(4):1595-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A Novel Population of alpha -Smooth Muscle Actin-Positive Cells Activated in a Rat Model of Stroke: An Analysis of the Spatio-Temporal Distribution in Response to Ischemia
Authors: Varun Sharma, Tina W Ling, Sarah S Rewell, David L Hare, David W Howells, Angela Kourakis et al.
Journal of Cerebral Blood Flow & Metabolism
-
Expression of cancer stem cell markers in pancreatic intraepithelial neoplasias and pancreatic ductal adenocarcinomas
Authors: SHOKO KURE, YOKO MATSUDA, MASAHITO HAGIO, JUNJI UEDA, ZENYA NAITO, TOSHIYUKI ISHIWATA
International Journal of Oncology
-
Phosphoproteome of Human Glioblastoma Initiating Cells Reveals Novel Signaling Regulators Encoded by the Transcriptome
Authors: Hiroko Kozuka-Hata, Yukiko Nasu-Nishimura, Ryo Koyama-Nasu, Hiroko Ao-Kondo, Kouhei Tsumoto, Tetsu Akiyama et al.
PLoS ONE
-
Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.
Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M
PLoS ONE, 2012-05-02;7(5):e35639.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
Stem Cells Dev., 2011-07-26;21(3):404-10.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes.
Authors: Engelen E, Akinci U, Bryne JC
Nat. Genet., 2011-05-01;43(6):607-11.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
An Engineered Cardiac Reporter Cell Line Identifies Human Embryonic Stem Cell-Derived Myocardial Precursors
Authors: Carissa Ritner, Sharon S. Y. Wong, Frank W. King, Shirley S. Mihardja, Walter Liszewski, David J. Erle et al.
PLoS ONE
-
Satellite cell heterogeneity with respect to expression of MyoD, myogenin, Dlk1 and c-Met in human skeletal muscle: application to a cohort of power lifters and sedentary men
Authors: Mona Lindström, Fatima Pedrosa-Domellöf, Lars-Eric Thornell
Histochemistry and Cell Biology
-
Timed inhibition of p38MAPK directs accelerated differentiation of human embryonic stem cells into cardiomyocytes
Authors: Meenakshi Gaur, Carissa Ritner, Rich Sievers, Anissa Pedersen, Megha Prasad, Harold S. Bernstein et al.
Cytotherapy
-
Expression of the stem cell markers nestin and CD133 on circulating melanoma cells.
Authors: Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U
J. Invest. Dermatol., 2010-09-30;131(2):487-94.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Subpopulations of Human Embryonic Stem Cells With Distinct Tissue-Specific Fates Can Be Selected From Pluripotent Cultures
Authors: Frank W. King, Carissa Ritner, Walter Liszewski, Helen C.K. Kwan, Anissa Pedersen, Andrew D. Leavitt et al.
Stem Cells and Development
-
Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey
Authors: Nobuhiro Shimozawa, Shinichiro Nakamura, Ichiro Takahashi, Masanori Hatori, Tadashi Sankai
REPRODUCTION
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Glial cell type-specific subcellular localization of 14-3-3 zeta: an implication for JCV tropism.
Authors: Lamba S, Ravichandran V, Major EO
Glia, 2009-07-01;57(9):971-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Induction of oligodendrogenesis in glioblastoma-initiating cells by IFN-mediated activation of STAT3 signaling.
Authors: Yuki K, Natsume A, Yokoyama H, Kondo Y, Ohno M, Kato T, Chansakul P, Ito M, Kim SU, Wakabayashi T
Cancer Lett., 2009-05-19;284(1):71-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
FEBS Lett., 2009-01-20;583(3):561-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures.
Authors: Vrana NE, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, Damour O, Hulmes DJ, Hasirci V
Invest. Ophthalmol. Vis. Sci., 2008-08-15;49(12):5325-31.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons.
Authors: Ladewig J, Koch P, Endl E, Meiners B, Opitz T, Couillard-Despres S, Aigner L, Brustle O
Stem Cells, 2008-04-17;26(7):1705-12.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The chemokine CXCL16 induces migration and invasion of glial precursor cells via its receptor CXCR6.
Authors: Hattermann K, Ludwig A, Gieselmann V, Held-Feindt J, Mentlein R
Mol. Cell. Neurosci., 2008-04-10;39(1):133-41.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture.
Authors: Sun Y, Pollard S, Conti L, Toselli M, Biella G, Parkin G, Willatt L, Falk A, Cattaneo E, Smith A
Mol. Cell. Neurosci., 2008-03-18;38(2):245-58.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The free-radical scavenger edaravone restores the differentiation of human neural precursor cells after radiation-induced oxidative stress.
Authors: Ishii J, Natsume A, Wakabayashi T, Takeuchi H, Hasegawa H, Kim SU, Yoshida J
Neurosci. Lett., 2007-08-03;423(3):225-30.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions.
Authors: Inagaki A, Soeda A, Oka N, Kitajima H, Nakagawa J, Motohashi T, Kunisada T, Iwama T
Biochem. Biophys. Res. Commun., 2007-07-18;361(3):586-92.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
Exp. Hematol., 2007-07-01;35(7):1119-31.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue.
Authors: Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD
Stem Cells, 2007-02-01;25(5):1298-306.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages.
Authors: Porat Y, Porozov S, Belkin D, Shimoni D, Fisher Y, Belleli A, Czeiger D, Silverman WF, Belkin M, Battler A, Fulga V, Savion N
Br. J. Haematol., 2006-12-01;135(5):703-14.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
FEBS Lett., 2006-10-02;580(25):5869-74.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Impaired proliferation and migration in human Miller-Dieker neural precursors.
Authors: Sheen VL, Ferland RJ, Harney M, Hill RS, Neal J, Banham AH, Brown P, Chenn A, Corbo J, Hecht J, Folkerth R, Walsh CA
Ann. Neurol., 2006-07-01;60(1):137-44.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions.
Authors: Shin S, Mitalipova M, Noggle S, Tibbitts D, Venable A, Rao R, Stice SL
Stem Cells, 2005-08-11;24(1):125-38.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells.
Authors: Gerecht-Nir S, Cohen S, Ziskind A, Itskovitz-Eldor J
Biotechnol. Bioeng., 2004-11-05;88(3):313-20.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
Brain Res. Dev. Brain Res., 2004-09-17;152(2):159-69.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Differentiation of human embryonic stem cells into insulin-producing clusters.
Authors: Segev H, Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J
Stem Cells, 2004-01-01;22(3):265-74.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virus
Authors: Thulasi Raman SN, Latreille E, Gao J et al.
Emerg Microbes Infect
-
Facile engineering of xeno-free microcarriers for the scalable cultivation of human pluripotent stem cells in stirred suspension.
Authors: Fan Y, Hsiung M, Cheng C et al.
Tissue Eng Part A.
-
Modelling and rescuing neurodevelopmental defect of Down syndrome using induced pluripotent stem cells from monozygotic twins discordant for trisomy 21.
Authors: Hibaoui Y, Grad I, Letourneau A et al.
EMBO Mol Med.
-
Human embryonic stem cell neural differentiation and enhanced cell survival promoted by hypoxic preconditioning.
Authors: Francis KR, Wei L.
Cell Death Dis
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Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: 5% non-fat milk in TBST
Dilution: 1/1000
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100