Human Neurocan Antibody

Catalog #: AF6508 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6508
AF6508-SP

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Human Neurocan Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neurocan in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Neurocan is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Neurocan
Glu23-Cys1321
Accession # O14594

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Neurocan in Human Brain. Neurocan was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6508) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurocan

Neurocan, also known as CSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that is primarily expressed in the central nervous system (1). Human Neurocan contains one Ig-like V-type domain, two Link domains, two EGF-like domains, one C‑type lectin-like domain, and one Sushi domain (2). It is an approximately 300 kDa molecule of which 66 kDa is chondroitin sulfate and 60 kDa is N‑ and O‑linked glycosylation (3). Mature human Neurocan shares 66% aa sequence identity with mature mouse and rat Neurocan. Human Neurocan is predicted to be cleaved following Met635, resulting in N-terminal (Neurocan-130) and C‑terminal (Neurocan-C) fragments whose core glycoproteins are 130 kDa and 150 kDa, respectively (4, 5). The full length molecule is expressed in the developing and juvenile brain, while its cleavage products are found throughout adulthood (3, 4). Neurocan and Neurocan-C are produced by astrocytes and accumulate in the matrix surrounding axonal bundles and neuronal cell bodies; Neurocan-130 is found mainly in the glial cell cytoplasm (6-8). Following brain injury, reactive astrocytes deposit increased amounts of Neurocan in the glial scar where it impedes axonal regeneration (6, 9). Neurocan inhibits neuronal adhesion and neurite outgrowth through interactions with a variety of matrix and transmembrane molecules including NCAM-L1, NCAM-1, Syndecan-3, Glypican-1, Tenascin, Contactin-2/TAG1, and HAPLN1 (1, 6, 10-15).

References

Rauch, U. et al. (2001) Cell. Mol. Life Sci. 58:1842.
Prange, C.K. et al. (1998) Gene 221:199.
Rauch, U. et al. (1991) J. Biol. Chem. 266:14785.
Matsui, F. et al. (1994) Neurochem. Int. 25:425.
Rauch, U. et al. (1992) J. Biol. Chem. 267:19536.
Asher, R.A. et al. (2000) J. Neurosci. 20:2427.
Matsui, F. et al. (1998) Brain Res. 790:45.
Abaskharoun, M. et al. (2010) Brain Res. 1327:6.
Shen, L.H. et al. (2008) Glia 56:1747.
Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
Sango, K. et al. (2003) Exp. Neurol. 182:1.
Akita, K. et al. (2004) Biochem. J. 383:129.
Grumet, M. et al. (1994) J. Biol. Chem. 269:12142.
Milev, P. et al. (1996) J. Biol. Chem. 271:15716.
Rauch, U. et al. (2004) Matrix Biol. 22:629.

Entrez Gene IDs

1463 (Human); 13004 (Mouse); 58982 (Rat)

Alternate Names

chondroitin sulfate proteoglycan 3 (neurocan); Chondroitin sulfate proteoglycan 3; CSPG3; CSPG3neurocan core protein; FLJ44681; NCAN; NEUR; neurocan proteoglycan; Neurocan