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Human Neurogenin-3 Antibody

Catalog #: AF3444
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3444
AF3444-SP
Detection of Human Neurogenin‑3 by Western Blot.
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Product Details
Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neurogenin-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Neurogenin-3
Met1-Leu214
Accession # Q9Y4Z2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human placenta

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Neurogenin‑3 antibody by Western Blot. View Larger

Detection of Human Neurogenin‑3 by Western Blot. Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurogenin-3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry View Larger

Neurogenin‑3 in Human Placenta. Neurogenin‑3 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell nuclei in decidual cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurogenin-3

Neurogenin-3 (NGN3; also Protein atonal homolog 5) is a 23 kDa, developmentally expressed nuclear protein that belongs to a family of atonal-related basic helix‑loop‑helix (bHLH) transcription factors. bHLH proteins typically form dimers when binding to DNA. NGN3 expression is required for the lineage differentiation of all endocrine cell types in the pancreas, including the formation of islet beta  cells. NGN3 is also neurogenic, controlling the number of dendrites generated by hippocampal neurons. Human NGN3 is 214 amino acids (aa) in length. It contains a DNA binding region (aa 84‑95) plus an HLH motif (aa 96‑136). Over aa 3‑214, human NGN3 shares 76% and 84% aa identity with mouse and canine NGN3, respectively.

Entrez Gene IDs
50674 (Human); 11925 (Mouse); 60329 (Rat)
Alternate Names
Atoh5; BHLHA7; bHLHa7NGN-3; Class A basic helix-loop-helix protein 7; Math4B; NEUROG3; neurogenin 3; Neurogenin3; Neurogenin-3; NGN3; Protein atonal homolog 5; Relax

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Citations for Human Neurogenin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster
    Authors: Miguel-Escalada I, Maestro MA, Balboa D et al.
    Developmental cell
  2. SIX2 Regulates Human &beta Cell Differentiation from Stem Cells and Functional Maturation In�Vitro
    Authors: L Velazco-Cr, MM Goedegebuu, KG Maxwell, P Augsornwor, NJ Hogrebe, JR Millman
    Cell Rep, 2020-05-26;31(8):107687.
  3. Characterization of cell-fate decision landscapes by estimating transcription factor dynamics
    Authors: Sara Jiménez, Valérie Schreiber, Reuben Mercier, Gérard Gradwohl, Nacho Molina
    Cell Reports Methods
  4. Gene Signatures of NEUROGENIN3+ Endocrine Progenitor Cells in the Human Pancreas
    Authors: Hyo Jeong Yong, Gengqiang Xie, Chengyang Liu, Wei Wang, Ali Naji, Jerome Irianto et al.
    Front Endocrinol (Lausanne)
  5. Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines
    Authors: NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman
    Nature Protocols, 2021-08-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  6. LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development
    Authors: NK Vinckier, NA Patel, RJ Geusz, A Wang, J Wang, I Matta, AR Harrington, M Wortham, N Wetton, J Wang, US Jhala, MG Rosenfeld, CW Benner, HP Shih, M Sander
    Nat Commun, 2020-04-29;11(1):2082.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Circadian Entrainment Triggers Maturation of Human In�Vitro Islets
    Authors: JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton
    Cell Stem Cell, 2019-12-12;26(1):108-122.e10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development
    Authors: N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton
    Cell Rep, 2019-05-21;27(8):2281-2291.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells
    Authors: EA Rosado-Oli, K Anderson, JH Kenty, DA Melton
    Nat Commun, 2019-04-01;10(1):1464.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC
  10. Insulin mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes
    Authors: Diego Balboa, Jonna Saarimäki-Vire, Daniel Borshagovski, Mantas Survila, Päivi Lindholm, Emilia Galli et al.
    eLife
  11. Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors
    Authors: II Aigha, B Memon, AK Elsayed, EM Abdelalim
    Stem Cell Res Ther, 2018-04-03;9(1):83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling
    Authors: Cyrille Ramond, Belin Selcen Beydag-Tasöz, Ajuna Azad, Martijn van de Bunt, Maja Borup Kjær Petersen, Nicola L. Beer et al.
    Development
  13. Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors
    Authors: NAJ Krentz, D van Hoof, Z Li, A Watanabe, M Tang, C Nian, MS German, FC Lynn
    Dev. Cell, 2017-04-24;41(2):129-142.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation
    Authors: J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski
    Cell Rep, 2017-04-11;19(2):281-294.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells.
    Authors: Cai, Qing, Bonfanti, Paola, Sambathkumar, Rangaraj, Vanuytsel, Kim, Vanhove, Jolien, Gysemans, Conny, Debiec-Rychter, Maria, Raitano, Susanna, Heimberg, Harry, Ordovas, Laura, Verfaillie, Catherin
    Stem Cells Transl Med, 2014-02-03;3(4):489-99.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Transplantation of Human Embryonic Stem Cell-Derived Pancreatic Endoderm Reveals a Site-Specific Survival, Growth, and Differentiation
    Authors: Lina Sui, Josué K. Mfopou, Bing Chen, Karen Sermon, Luc Bouwens
    Cell Transplantation
  17. The SDF-1α/CXCR4 Axis is Required for Proliferation and Maturation of Human Fetal Pancreatic Endocrine Progenitor Cells.
    Authors: Kayali AG, Lopez AD, Hao E, Hinton A, Hayek A, King CC
    PLoS ONE, 2012-06-22;7(6):e38721.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
    Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Nat. Biotechnol., 2020-02-24;0(0):.
  19. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
    Authors: B Memon, M Karam, S Al-Khawaga, EM Abdelalim
    Stem Cell Res Ther, 2018-01-23;9(1):15.

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