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Human Neuromedin BR/NMBR Antibody

Catalog #: MAB4728 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4728
MAB4728-SP
Detection of Neuromedin B R/ NMBR in HT‑29 Human Cell Line by Flow Cytometry.
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Human Neuromedin BR/NMBR Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neuromedin B R/NMBR. Stains human Neuromedin B R/NMBR transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 466505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human NMBR
Met1-Met390
Accession # AAB27330
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Neuromedin B R/ NMBR antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Neuromedin B R/ NMBR in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB4728, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Flow Cytometry Detection of Neuromedin B R/NMBR antibody in HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP antibody by Flow Cytometry. View Larger

Detection of Neuromedin B R/NMBR in HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) Neuromedin B R/NMBR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB4728) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuromedin BR/NMBR

Neuromedin B Receptor (NMBR) is an 80 kDa, 390 amino acid (aa) G-protein coupled 7-transmembrane glycoprotein receptor for bombesin-like peptides, binding Neuromedin B with high affinity and GRP (gastrin releasing peptide) with lower affinity.  Neuromedin B R expression in the olfactory and central thalamic regions of the brain plays a role in thermoregulation. It has also been shown to be mitogenic in colonic epithelium. Expression in the pituitary gland is important for regulation of the pituitary-thyroid axis. Within extracellular domains, human Neuromedin B R shares 86% and 82% aa identity with mouse and rat Neuromedin B R, respectively.

Long Name
Neuromedin B Receptor
Entrez Gene IDs
4829 (Human); 18101 (Mouse); 25264 (Rat)
Alternate Names
Neuromedin B R; neuromedin B receptor; Neuromedin BR; neuromedin-B receptor; Neuromedin-B-preferring bombesin receptor; NMBR; NMB-R

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