Human Neuropeptide Y/NPY Antibody Summary
Accession # P01303
Applications
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Neuropeptide Y/NPY Monoclonal Antibody (Catalog # MAB85172).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Neuropeptide Y/NPY DuoSet ELISA Kit (Catalog # DY8517-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Human Neuropeptide Y/NPY ELISA Standard Curve. Recombinant Human Neuropeptide Y/NPY protein was serially diluted 2-fold and captured by Mouse Anti-Human Neuropeptide Y/NPY Monoclonal Antibody (Catalog # MAB85171) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Neuropeptide Y/NPY Monoclonal Antibody (Catalog # MAB85172) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuropeptide Y/NPY
Neuropeptide Y (NPY) is a 36 amino acid peptide that was isolated from hypothalamus in porcine brain in 1982 and lately it belongs to a family of peptides which include Pancreatic Polypeptide (PP) and Peptide YY (PYY) which exert their pharmacological action via interaction with G-protein coupled receptors Y1, Y2, Y4, Y5 and y6. NPY is the most abundant peptide in brain and in nervous system NPY functions as a neurotransmitter regulating many processes including memory and learning, pain, fat storage and blood pressure. NPY also regulates stress by stimulating secretion of corticotropin-releasing hormone in brain. It appears there is a correlation between the increased levels of NPY gene expression in hippocampus and epileptic seizures. Cocaine reduces the levels of NPY and such a decrease is thought to be related to depression and anxiety. NPY receptors are rhodopsin-like G-protein coupled receptors (GPCR) coupled to Gi or G0 proteins, which inhibit adenylate cyclase and reduce cAMP accumulation and modulate Calcium and Potassium channels.
Product Datasheets
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