Human Neurotrimin Biotinylated Antibody

Catalog #: BAF1235 Datasheet / COA / SDS

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BAF1235

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Human Neurotrimin Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neurotrimin in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Neurotrimin
Val31-Asn321
Accession # Q9P121

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Neurotrimin

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Neurotrimin in Human Spinal Cord. Neurotrimin was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human Neurotrimin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1235) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurotrimin

Neurotrimin is in a glycosyl phosphatidyl inositol (GPI)-anchored cell adhesion molecule on neuronal populations. It is a member of the IgLON (immunoglobulin superfamily containing LAMP, OBCAM and Neurotrimin) subfamily of the immunoglobulin superfamily.

Entrez Gene IDs

50863 (Human)

Alternate Names

HNT; IgLON family member 2; IGLON2HNTNT; MGC60329; Neurotrimin; NTRI